Scanning tunnelling microscopy observations of biomolecules on layered materials

Scanning tunnelling microscopy (STM) has been performed on the reverse transcriptases of the human immunodeficiency virus (HIV-1) and the moloney murine leukaemia virus (MuLV). The biological molecules are adsorbed on n-type semiconducting MoTe2. The p66 (66 kD) subunit of the RT of HIV-1 is imaged...

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Bibliographic Details
Published in:Faraday discussions 1992, Vol.94 (94), p.183-197
Main Authors: Jungblut, H, Campbell, S A, Giersig, M, Müller, D J, Lewerenz, H J
Format: Article
Language:English
Subjects:
AIDS/HIV
Dialysis
HIV Reverse Transcriptase
HIV-1 - enzymology
Microscopy, Scanning Tunneling
Moloney murine leukemia virus - enzymology
Neutrons
RNA-Directed DNA Polymerase - analysis
Scattering, Radiation
Spectrophotometry, Ultraviolet
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Summary:Scanning tunnelling microscopy (STM) has been performed on the reverse transcriptases of the human immunodeficiency virus (HIV-1) and the moloney murine leukaemia virus (MuLV). The biological molecules are adsorbed on n-type semiconducting MoTe2. The p66 (66 kD) subunit of the RT of HIV-1 is imaged by STM. Both STM and processed transmission electron microscopy (TEM) data show a spherical and horseshoe-like shape of external diameter ca. 65 A, depending on the angle of observation. The STM results show a larger diameter which is related to the curvature radius of the tip of the probing needle. The RTs of HIV-1 and MuLV exhibit a circular hole of ca. 20 A diameter in accordance with structure predictions and functioning considerations. The surface-molecule interaction is discussed in terms of the electronic properties of the semiconductor surface including the influence of small defect sites at the layered crystal surface.
ISSN:1359-6640
1364-5498
DOI:10.1039/FD9929400183