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Trafficking of galectin-3 through endosomal organelles of polarized and non-polarized cells

In epithelial cells, the β-galactoside-binding lectin galectin-3 mediates the non-raft-dependent glycoprotein targeting to the apical membrane domain. In this study, we aimed to identify intracellular compartments involved in the trafficking of galectin-3. By studying fluorescent fusion proteins in...

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Published in:	European journal of cell biology 2010-11, Vol.89 (11), p.788-798
Main Authors:	Schneider, Dominik, Greb, Christoph, Koch, Annett, Straube, Tamara, Elli, Alexandra, Delacour, Delphine, Jacob, Ralf
Format:	Article
Language:	English
Subjects:	Animals Apical trafficking Cell Line Cell Membrane - metabolism Cell Polarity - genetics Cercopithecus aethiops COS Cells COS-1 Dogs Endosomes Endosomes - genetics Endosomes - metabolism Epithelial Cells - cytology Epithelial Cells - metabolism Galectin 3 - genetics Galectin 3 - metabolism Galectin-3 MDCK Microscopy, Fluorescence Organelles - genetics Organelles - metabolism Protein Transport Sorting
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creator	Schneider, Dominik Greb, Christoph Koch, Annett Straube, Tamara Elli, Alexandra Delacour, Delphine Jacob, Ralf
description	In epithelial cells, the β-galactoside-binding lectin galectin-3 mediates the non-raft-dependent glycoprotein targeting to the apical membrane domain. In this study, we aimed to identify intracellular compartments involved in the trafficking of galectin-3. By studying fluorescent fusion proteins in living cells, we could show that galectin-3 accumulates intracellularly in acidified endosomes. Total internal reflection fluorescence microscopy studies of the apical surface of polarized MDCK cells revealed that galectin-3 is enriched in tubular and vesicular Rab11-positive recycling endosomes in the vicinity of the apical cell surface. These endosomal organelles are candidate compartments for the association between galectin-3 and exocytic apical cargo.
doi_str_mv	10.1016/j.ejcb.2010.07.001
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subjects	Animals Apical trafficking Cell Line Cell Membrane - metabolism Cell Polarity - genetics Cercopithecus aethiops COS Cells COS-1 Dogs Endosomes Endosomes - genetics Endosomes - metabolism Epithelial Cells - cytology Epithelial Cells - metabolism Galectin 3 - genetics Galectin 3 - metabolism Galectin-3 MDCK Microscopy, Fluorescence Organelles - genetics Organelles - metabolism Protein Transport Sorting
title	Trafficking of galectin-3 through endosomal organelles of polarized and non-polarized cells
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