Bone Marrow Stem Cell Transplantation to Olfactory Epithelium

Objectives: We sought to develop a new therapeutic strategy for degeneration of olfactory receptor neurons (ORNs). Methods: We transplanted into Balb/C mice, locally by transnasal injection and systemically via the tail vain, BrdU-labeled bone marrow stem cells, also known as NRGs, which have the ab...

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Bibliographic Details
Published in:Annals of otology, rhinology & laryngology rhinology & laryngology, 2010-08, Vol.119 (8), p.535-540
Main Authors: Ochi, Naoki, Doi, Kiyoshi, Uranagase, Masahiro, Nishikawa, Tasuku, Katsunuma, Sayaka, Nibu, Ken-Ichi
Format: Article
Language:English
Subjects:
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Animals
Biological and medical sciences
Bone Marrow Transplantation - methods
Bone marrow, stem cells transplantation. Graft versus host reaction
Cell Movement
Disease Models, Animal
GAP-43 Protein - metabolism
Male
Medical sciences
Mice
Mice, Inbred BALB C
Nerve Degeneration - therapy
Olfactory Bulb
Olfactory Marker Protein - metabolism
Olfactory Mucosa
Otorhinolaryngology. Stomatology
Stem Cell Transplantation - methods
Transfusions. Complications. Transfusion reactions. Cell and gene therapy
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Summary:Objectives: We sought to develop a new therapeutic strategy for degeneration of olfactory receptor neurons (ORNs). Methods: We transplanted into Balb/C mice, locally by transnasal injection and systemically via the tail vain, BrdU-labeled bone marrow stem cells, also known as NRGs, which have the ability to differentiate into neural cells. Bone marrow stem cells engrafted into the olfactory epithelium were examined immunohistochemically. Results: Compared with previous studies, in which bone marrow was transplanted rather than bone marrow stem cells, migration of transplanted bone marrow stem cells into the olfactory epithelium was observed earlier, and engraftment rates were significantly higher. However, migrated bone marrow stem cells were positive for GAP43 but not for olfactory marker protein. Conclusions: These results suggest that engrafted cells had differentiated into premature, but not mature, ORNs. Further experiments using autologous bone marrow stem cells in combination with various growth factors and/or neurotrophic factors should aid the development of new therapeutic methods for degenerated ORNs.
ISSN:0003-4894
1943-572X
DOI:10.1177/000348941011900806