Heat-killed Lactobacillus rhamnosus GG Modulates Urocortin and Cytokine Release in Primary Trophoblast Cells

Abstract A number of studies are showing that probiotic treatment induces an anti-inflammatory state. Intrauterine infection can lead to preterm delivery by modulating immune function and efforts to prevent this condition are ongoing nowadays. Lactobacillus rhamnosus GG (LGG) is a probiotic known to...

Full description

Saved in:
Bibliographic Details
Published in:Placenta (Eastbourne) 2010-10, Vol.31 (10), p.867-872
Main Authors: Bloise, E, Torricelli, M, Novembri, R, Borges, L.E, Carrarelli, P, Reis, F.M, Petraglia, F
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chorionic Gonadotropin - immunology
Corticotropin-Releasing Hormone - immunology
Cytokines
Cytokines - genetics
Cytokines - immunology
Embryology: invertebrates and vertebrates. Teratology
Enzyme-Linked Immunosorbent Assay
Female
Fundamental and applied biological sciences. Psychology
Humans
Internal Medicine
Lactobacilli
Lactobacillus rhamnosus - immunology
Obstetrics and Gynecology
Placenta
Placenta - cytology
Placenta - immunology
Placenta - microbiology
Pregnancy
Preterm delivery
Probiotics - pharmacology
Progesterone - immunology
Reverse Transcriptase Polymerase Chain Reaction
RNA - chemistry
RNA - genetics
Trophoblasts - cytology
Trophoblasts - immunology
Urocortin
Urocortins - genetics
Urocortins - immunology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract A number of studies are showing that probiotic treatment induces an anti-inflammatory state. Intrauterine infection can lead to preterm delivery by modulating immune function and efforts to prevent this condition are ongoing nowadays. Lactobacillus rhamnosus GG (LGG) is a probiotic known to ameliorate inflammation by increasing local anti-inflammatory mediators in urinary and gastrointestinal tracts. The present study then analyzed the effect of heat-killed LGG over β-hCG, progesterone, interleukins (IL) 4 and 10, tumor necrosis factor-α (TNF-α), corticotropin releasing hormone (CRH) and urocortin (Ucn) release by primary trophoblast cells. Normal human term placentas (n = 6) were collected and purified trophoblast cells were incubated in the presence of LGG, lipopolysaccharide (LPS) or either LGG + LPS during 3 h, after which the target substances were quantified by ELISA and real-time PCR. LGG did not affect β-hCG, progesterone, or CRH secretion. Conversely, LGG increased IL-4 protein and mRNA expression ( P  
ISSN:0143-4004
1532-3102
DOI:10.1016/j.placenta.2010.04.007