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Heat-killed Lactobacillus rhamnosus GG Modulates Urocortin and Cytokine Release in Primary Trophoblast Cells
Abstract A number of studies are showing that probiotic treatment induces an anti-inflammatory state. Intrauterine infection can lead to preterm delivery by modulating immune function and efforts to prevent this condition are ongoing nowadays. Lactobacillus rhamnosus GG (LGG) is a probiotic known to...
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| Published in: | Placenta (Eastbourne) 2010-10, Vol.31 (10), p.867-872 |
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| cites | cdi_FETCH-LOGICAL-c518t-82e2754c7b40686be8e82a8472ae22e5db2474859c3a17830001794988d480023 |
| container_end_page | 872 |
| container_issue | 10 |
| container_start_page | 867 |
| container_title | Placenta (Eastbourne) |
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| creator | Bloise, E Torricelli, M Novembri, R Borges, L.E Carrarelli, P Reis, F.M Petraglia, F |
| description | Abstract A number of studies are showing that probiotic treatment induces an anti-inflammatory state. Intrauterine infection can lead to preterm delivery by modulating immune function and efforts to prevent this condition are ongoing nowadays. Lactobacillus rhamnosus GG (LGG) is a probiotic known to ameliorate inflammation by increasing local anti-inflammatory mediators in urinary and gastrointestinal tracts. The present study then analyzed the effect of heat-killed LGG over β-hCG, progesterone, interleukins (IL) 4 and 10, tumor necrosis factor-α (TNF-α), corticotropin releasing hormone (CRH) and urocortin (Ucn) release by primary trophoblast cells. Normal human term placentas (n = 6) were collected and purified trophoblast cells were incubated in the presence of LGG, lipopolysaccharide (LPS) or either LGG + LPS during 3 h, after which the target substances were quantified by ELISA and real-time PCR. LGG did not affect β-hCG, progesterone, or CRH secretion. Conversely, LGG increased IL-4 protein and mRNA expression ( P |
| doi_str_mv | 10.1016/j.placenta.2010.04.007 |
| format | article |
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Intrauterine infection can lead to preterm delivery by modulating immune function and efforts to prevent this condition are ongoing nowadays. Lactobacillus rhamnosus GG (LGG) is a probiotic known to ameliorate inflammation by increasing local anti-inflammatory mediators in urinary and gastrointestinal tracts. The present study then analyzed the effect of heat-killed LGG over β-hCG, progesterone, interleukins (IL) 4 and 10, tumor necrosis factor-α (TNF-α), corticotropin releasing hormone (CRH) and urocortin (Ucn) release by primary trophoblast cells. Normal human term placentas (n = 6) were collected and purified trophoblast cells were incubated in the presence of LGG, lipopolysaccharide (LPS) or either LGG + LPS during 3 h, after which the target substances were quantified by ELISA and real-time PCR. LGG did not affect β-hCG, progesterone, or CRH secretion. Conversely, LGG increased IL-4 protein and mRNA expression ( P < 0.05) while IL-10 and Ucn secretion were increased in a dose dependent manner and the highest dose of LGG increased significantly IL-10 mRNA ( P < 0.05). LGG did not alter TNF-α, while LPS exposure increased TNF-α protein ( P < 0.001) and mRNA expression ( P < 0.01). Conversely, LGG treatment reversed LPS-induced TNF-α release at both protein ( P < 0.01) and mRNA levels ( P < 0.05) in a dose dependent fashion. In conclusion, LGG stimulates IL-4, IL-10 and Ucn expression and reverses LPS-induced TNF-α release from trophoblast cells, with no change in β-hCG or progesterone release, suggesting that this probiotic may play a role as an immunomodulatory agent in human placenta without altering basic trophoblast functions.]]></description><identifier>ISSN: 0143-4004</identifier><identifier>EISSN: 1532-3102</identifier><identifier>DOI: 10.1016/j.placenta.2010.04.007</identifier><identifier>PMID: 20696472</identifier><identifier>CODEN: PLACDF</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Biological and medical sciences ; Chorionic Gonadotropin - immunology ; Corticotropin-Releasing Hormone - immunology ; Cytokines ; Cytokines - genetics ; Cytokines - immunology ; Embryology: invertebrates and vertebrates. Teratology ; Enzyme-Linked Immunosorbent Assay ; Female ; Fundamental and applied biological sciences. Psychology ; Humans ; Internal Medicine ; Lactobacilli ; Lactobacillus rhamnosus - immunology ; Obstetrics and Gynecology ; Placenta ; Placenta - cytology ; Placenta - immunology ; Placenta - microbiology ; Pregnancy ; Preterm delivery ; Probiotics - pharmacology ; Progesterone - immunology ; Reverse Transcriptase Polymerase Chain Reaction ; RNA - chemistry ; RNA - genetics ; Trophoblasts - cytology ; Trophoblasts - immunology ; Urocortin ; Urocortins - genetics ; Urocortins - immunology</subject><ispartof>Placenta (Eastbourne), 2010-10, Vol.31 (10), p.867-872</ispartof><rights>2010</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010. Published by Elsevier Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c518t-82e2754c7b40686be8e82a8472ae22e5db2474859c3a17830001794988d480023</citedby><cites>FETCH-LOGICAL-c518t-82e2754c7b40686be8e82a8472ae22e5db2474859c3a17830001794988d480023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23303130$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20696472$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bloise, E</creatorcontrib><creatorcontrib>Torricelli, M</creatorcontrib><creatorcontrib>Novembri, R</creatorcontrib><creatorcontrib>Borges, L.E</creatorcontrib><creatorcontrib>Carrarelli, P</creatorcontrib><creatorcontrib>Reis, F.M</creatorcontrib><creatorcontrib>Petraglia, F</creatorcontrib><title>Heat-killed Lactobacillus rhamnosus GG Modulates Urocortin and Cytokine Release in Primary Trophoblast Cells</title><title>Placenta (Eastbourne)</title><addtitle>Placenta</addtitle><description><![CDATA[Abstract A number of studies are showing that probiotic treatment induces an anti-inflammatory state. Intrauterine infection can lead to preterm delivery by modulating immune function and efforts to prevent this condition are ongoing nowadays. Lactobacillus rhamnosus GG (LGG) is a probiotic known to ameliorate inflammation by increasing local anti-inflammatory mediators in urinary and gastrointestinal tracts. The present study then analyzed the effect of heat-killed LGG over β-hCG, progesterone, interleukins (IL) 4 and 10, tumor necrosis factor-α (TNF-α), corticotropin releasing hormone (CRH) and urocortin (Ucn) release by primary trophoblast cells. Normal human term placentas (n = 6) were collected and purified trophoblast cells were incubated in the presence of LGG, lipopolysaccharide (LPS) or either LGG + LPS during 3 h, after which the target substances were quantified by ELISA and real-time PCR. LGG did not affect β-hCG, progesterone, or CRH secretion. Conversely, LGG increased IL-4 protein and mRNA expression ( P < 0.05) while IL-10 and Ucn secretion were increased in a dose dependent manner and the highest dose of LGG increased significantly IL-10 mRNA ( P < 0.05). LGG did not alter TNF-α, while LPS exposure increased TNF-α protein ( P < 0.001) and mRNA expression ( P < 0.01). Conversely, LGG treatment reversed LPS-induced TNF-α release at both protein ( P < 0.01) and mRNA levels ( P < 0.05) in a dose dependent fashion. In conclusion, LGG stimulates IL-4, IL-10 and Ucn expression and reverses LPS-induced TNF-α release from trophoblast cells, with no change in β-hCG or progesterone release, suggesting that this probiotic may play a role as an immunomodulatory agent in human placenta without altering basic trophoblast functions.]]></description><subject>Biological and medical sciences</subject><subject>Chorionic Gonadotropin - immunology</subject><subject>Corticotropin-Releasing Hormone - immunology</subject><subject>Cytokines</subject><subject>Cytokines - genetics</subject><subject>Cytokines - immunology</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Internal Medicine</subject><subject>Lactobacilli</subject><subject>Lactobacillus rhamnosus - immunology</subject><subject>Obstetrics and Gynecology</subject><subject>Placenta</subject><subject>Placenta - cytology</subject><subject>Placenta - immunology</subject><subject>Placenta - microbiology</subject><subject>Pregnancy</subject><subject>Preterm delivery</subject><subject>Probiotics - pharmacology</subject><subject>Progesterone - immunology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA - chemistry</subject><subject>RNA - genetics</subject><subject>Trophoblasts - cytology</subject><subject>Trophoblasts - immunology</subject><subject>Urocortin</subject><subject>Urocortins - genetics</subject><subject>Urocortins - immunology</subject><issn>0143-4004</issn><issn>1532-3102</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqFkt-LEzEQx4MoXq_6Lxx5EZ-2Tn7sbvoiStHeQUXRu-eQzU65tGlSk12h_71Z2lPwxYeQzPCdmW8-DCE3DBYMWPNutzh6YzEMZsGhJEEuANpnZMZqwSvBgD8nM2BSVBJAXpHrnHcAsJSMvyRXHJplI1s-I_4WzVDtnffY042xQ-yMLdGYaXo0hxBzea3X9EvsR28GzPQhRRvT4AI1oaer0xD3LiD9jh5NRlry35I7mHSi9ykeH2PnTR7oCr3Pr8iLrfEZX1_uOXn4_Ol-dVttvq7vVh83la2ZGirFkbe1tG0noVFNhwoVN6r4Ncg51n3HZStVvbTCsFaJ8i_WLuVSqV4qAC7m5O257zHFnyPmQR9ctsWBCRjHrNu6LnVSiKJszkqbYs4Jt_p4Nq8Z6Am03ukn0HoCrUHqAroU3lxGjN0B-z9lT2SL4M1FYLI1fptMsC7_1QkBgpUzJx_OOixAfjlMOluHwWLvEtpB99H938v7f1pY74IrU_d4wryLYwoFt2Y6cw36x7QW01awCVxdAP4GhAGzOQ</recordid><startdate>20101001</startdate><enddate>20101001</enddate><creator>Bloise, E</creator><creator>Torricelli, M</creator><creator>Novembri, R</creator><creator>Borges, L.E</creator><creator>Carrarelli, P</creator><creator>Reis, F.M</creator><creator>Petraglia, F</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20101001</creationdate><title>Heat-killed Lactobacillus rhamnosus GG Modulates Urocortin and Cytokine Release in Primary Trophoblast Cells</title><author>Bloise, E ; Torricelli, M ; Novembri, R ; Borges, L.E ; Carrarelli, P ; Reis, F.M ; Petraglia, F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c518t-82e2754c7b40686be8e82a8472ae22e5db2474859c3a17830001794988d480023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Biological and medical sciences</topic><topic>Chorionic Gonadotropin - immunology</topic><topic>Corticotropin-Releasing Hormone - immunology</topic><topic>Cytokines</topic><topic>Cytokines - genetics</topic><topic>Cytokines - immunology</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Internal Medicine</topic><topic>Lactobacilli</topic><topic>Lactobacillus rhamnosus - immunology</topic><topic>Obstetrics and Gynecology</topic><topic>Placenta</topic><topic>Placenta - cytology</topic><topic>Placenta - immunology</topic><topic>Placenta - microbiology</topic><topic>Pregnancy</topic><topic>Preterm delivery</topic><topic>Probiotics - pharmacology</topic><topic>Progesterone - immunology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA - chemistry</topic><topic>RNA - genetics</topic><topic>Trophoblasts - cytology</topic><topic>Trophoblasts - immunology</topic><topic>Urocortin</topic><topic>Urocortins - genetics</topic><topic>Urocortins - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bloise, E</creatorcontrib><creatorcontrib>Torricelli, M</creatorcontrib><creatorcontrib>Novembri, R</creatorcontrib><creatorcontrib>Borges, L.E</creatorcontrib><creatorcontrib>Carrarelli, P</creatorcontrib><creatorcontrib>Reis, F.M</creatorcontrib><creatorcontrib>Petraglia, F</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Placenta (Eastbourne)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bloise, E</au><au>Torricelli, M</au><au>Novembri, R</au><au>Borges, L.E</au><au>Carrarelli, P</au><au>Reis, F.M</au><au>Petraglia, F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Heat-killed Lactobacillus rhamnosus GG Modulates Urocortin and Cytokine Release in Primary Trophoblast Cells</atitle><jtitle>Placenta (Eastbourne)</jtitle><addtitle>Placenta</addtitle><date>2010-10-01</date><risdate>2010</risdate><volume>31</volume><issue>10</issue><spage>867</spage><epage>872</epage><pages>867-872</pages><issn>0143-4004</issn><eissn>1532-3102</eissn><coden>PLACDF</coden><abstract><![CDATA[Abstract A number of studies are showing that probiotic treatment induces an anti-inflammatory state. Intrauterine infection can lead to preterm delivery by modulating immune function and efforts to prevent this condition are ongoing nowadays. Lactobacillus rhamnosus GG (LGG) is a probiotic known to ameliorate inflammation by increasing local anti-inflammatory mediators in urinary and gastrointestinal tracts. The present study then analyzed the effect of heat-killed LGG over β-hCG, progesterone, interleukins (IL) 4 and 10, tumor necrosis factor-α (TNF-α), corticotropin releasing hormone (CRH) and urocortin (Ucn) release by primary trophoblast cells. Normal human term placentas (n = 6) were collected and purified trophoblast cells were incubated in the presence of LGG, lipopolysaccharide (LPS) or either LGG + LPS during 3 h, after which the target substances were quantified by ELISA and real-time PCR. LGG did not affect β-hCG, progesterone, or CRH secretion. Conversely, LGG increased IL-4 protein and mRNA expression ( P < 0.05) while IL-10 and Ucn secretion were increased in a dose dependent manner and the highest dose of LGG increased significantly IL-10 mRNA ( P < 0.05). LGG did not alter TNF-α, while LPS exposure increased TNF-α protein ( P < 0.001) and mRNA expression ( P < 0.01). Conversely, LGG treatment reversed LPS-induced TNF-α release at both protein ( P < 0.01) and mRNA levels ( P < 0.05) in a dose dependent fashion. In conclusion, LGG stimulates IL-4, IL-10 and Ucn expression and reverses LPS-induced TNF-α release from trophoblast cells, with no change in β-hCG or progesterone release, suggesting that this probiotic may play a role as an immunomodulatory agent in human placenta without altering basic trophoblast functions.]]></abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>20696472</pmid><doi>10.1016/j.placenta.2010.04.007</doi><tpages>6</tpages></addata></record> |
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| subjects | Biological and medical sciences Chorionic Gonadotropin - immunology Corticotropin-Releasing Hormone - immunology Cytokines Cytokines - genetics Cytokines - immunology Embryology: invertebrates and vertebrates. Teratology Enzyme-Linked Immunosorbent Assay Female Fundamental and applied biological sciences. Psychology Humans Internal Medicine Lactobacilli Lactobacillus rhamnosus - immunology Obstetrics and Gynecology Placenta Placenta - cytology Placenta - immunology Placenta - microbiology Pregnancy Preterm delivery Probiotics - pharmacology Progesterone - immunology Reverse Transcriptase Polymerase Chain Reaction RNA - chemistry RNA - genetics Trophoblasts - cytology Trophoblasts - immunology Urocortin Urocortins - genetics Urocortins - immunology |
| title | Heat-killed Lactobacillus rhamnosus GG Modulates Urocortin and Cytokine Release in Primary Trophoblast Cells |
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