Evaluation of proliferating cell nuclear antigen (PCNA) as an endogenous marker of cell proliferation in rat liver: a dual-stain comparison with 5-bromo-2'-deoxyuridine

Proliferating cell nuclear antigen (PCNA) was evaluated as a marker of cell proliferation in formalin-fixed rat liver tissue through a comparative study with the thymidine analogue 5-bromo-2'-deoxyuridine (BrdU). The comparison was conducted through the introduction of a dual immunohistochemica...

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Bibliographic Details
Published in:The journal of histochemistry and cytochemistry 1993-01, Vol.41 (1), p.1-6
Main Authors: Connolly, KM, Bogdanffy, MS
Format: Article
Language:English
Subjects:
Animals
Biomarkers - analysis
Bromodeoxyuridine
Cell Division
G1 Phase
Immunohistochemistry - methods
Liver - cytology
Nuclear Proteins - analysis
Proliferating Cell Nuclear Antigen
Rats
Rats, Inbred Strains
S Phase
Staining and Labeling
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Summary:Proliferating cell nuclear antigen (PCNA) was evaluated as a marker of cell proliferation in formalin-fixed rat liver tissue through a comparative study with the thymidine analogue 5-bromo-2'-deoxyuridine (BrdU). The comparison was conducted through the introduction of a dual immunohistochemical procedure that allows the simultaneous detection of the two antigens. The results of this study suggest that although statistically similar indexes for each can be achieved, what has been reported to be the "S-phase fraction" of PCNA-labeled nuclei is significantly different from the population of cells marked by BrdU. The data also suggest that the reason for this difference is that the "S-phase fraction" of PCNA-labeled nuclei is the population of cells in late G1- and early S-phases. BrdU, by comparison, is incorporated into cells only during DNA synthesis. Therefore, although BrdU and PCNA labeling techniques may both be effective for evaluating cell proliferation rates, it must be recognized that labeling indices derived from each are not entirely synonymous. The method presented here for the simultaneous labeling of PCNA and BrdU antigens may have utility in studies of cell cycle perturbations.
ISSN:0022-1554
1551-5044
DOI:10.1177/41.1.7678022