Molecular Epidemiology of Type 1 Polioviruses Isolated in Israel and Defined by Restriction Fragment Length Polymorphism Assay

The genomic variability of 27 type 1 polioviruses (PV-1) isolated in Israel during 1980–1991 was examined by restriction fragment length polymorphism (RFLP) analysis of a reverse-transcribed genomic fragment amplified by polymerase chain reaction. By using the restriction enzymes HaeIII, DdeI, and H...

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Bibliographic Details
Published in:The Journal of infectious diseases 1993-01, Vol.167 (1), p.199-203
Main Authors: Vonsover, Ami, Handsher, Rachel, Neuman, Mina, Guillot, Sophie, Balanant, Jean, Rudich, Hagit, Mendelson, Ella, Swartz, Tiberius, Crainic, Radu
Format: Article
Language:English
Subjects:
Base Sequence
Complementary DNA
Concise Communications
DNA
Enzymes
Genome, Viral
Genomics
Humans
Israel - epidemiology
Molecular Sequence Data
Nucleotide sequences
Poliomyelitis
Poliomyelitis - epidemiology
Poliomyelitis - microbiology
Poliovirus
Poliovirus - genetics
poliovirus 1
Polymerase chain reaction
Polymorphism, Restriction Fragment Length
Restriction fragment length polymorphism
Sequence Analysis, DNA
Viruses
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Summary:The genomic variability of 27 type 1 polioviruses (PV-1) isolated in Israel during 1980–1991 was examined by restriction fragment length polymorphism (RFLP) analysis of a reverse-transcribed genomic fragment amplified by polymerase chain reaction. By using the restriction enzymes HaeIII, DdeI, and HpaII, strain-specific restriction profiles were generated for the PV-l/Mahoney and PV-1/Sabin strains and 27 wild-type isolates. The profile observed for PV-l isolated during an outbreak in 1988 was also observed for PV-1 isolated from different places in Israel in 1982 and 1983, 1987, and 1991. This profile, characterized by the lack of the DdeI site, was different from the DdeI profile of PV-1 isolated in 1984 or in 1986 from sporadic cases of poliomyelitis. The diversity of circulating PV-1 in Israel was also confirmed by nucleotide sequence analysis. The epidemiologic information provided by the RFLP and sequence data establishes a clear epidemiologic link between epidemic and sporadic virus strains and demonstrates the power of this molecular approach to epidemiology.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/167.1.199