Ethanol-induced inhibition of glucose transport across the isolated brush-border membrane of hamster jejunum

Acute exposure of jejunal mucosa to ethanol has been reported to produce a depression of transmural glucose transport across this organ in vitro and in vivo. In an attempt to understand the mechanism of action of ethanol on intestinal transport, in the present study we have investigated the effect o...

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Bibliographic Details
Published in:Digestive diseases and sciences 1981-01, Vol.26 (1), p.23-32
Main Authors: Dinda, P K, Beck, I T
Format: Article
Language:English
Subjects:
Animals
Biological Transport - drug effects
Cell Membrane - drug effects
Cell Membrane - metabolism
Cell Membrane - ultrastructure
Cell Membrane Permeability - drug effects
Cricetinae
Ethanol - pharmacology
Glucose - metabolism
In Vitro Techniques
Jejunum - drug effects
Jejunum - metabolism
Jejunum - ultrastructure
Mannose - metabolism
Membrane Proteins - analysis
Mesocricetus
Microvilli - drug effects
Microvilli - metabolism
Microvilli - ultrastructure
Sodium - metabolism
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Summary:Acute exposure of jejunal mucosa to ethanol has been reported to produce a depression of transmural glucose transport across this organ in vitro and in vivo. In an attempt to understand the mechanism of action of ethanol on intestinal transport, in the present study we have investigated the effect of ethanol on glucose uptake by purified brush-border membrane vesicles of hamster jejunum. Ethanol, in concentrations found in man after moderate drinking (1-5% w/v), was found to depress glucose uptake by the brush-border membrane in a dose-dependent and time-dependent manner. Mannose was used to measure nonspecific uptake, and we found that the ethanol-induced depression of glucose uptake was not related to an alteration of the nonspecific uptake of this sugar. The inhibition of glucose uptake of the ethanol-treated membranes completely disappeared after repeated washing of the membranes with ethanol-free buffer. Accordingly, the ethanol-induced depression of glucose uptake was not the result of irreversible damage to membrane proteins but was related to a direct effect of ethanol on the brush-border membrane. On the basis of these findings, it is concluded that a direct interference with glucose translocation across the brush border plays an important role in the ethanol-induced depression of transmural jejunal glucose absorption.
ISSN:0163-2116
1573-2568
DOI:10.1007/BF01307972