The herpes simplex virus type-1 origin binding protein. DNA helicase activity

The herpes simplex virus type 1 (HSV-1) origin binding protein, the product of the UL9 gene, catalyzes the unwinding of DNA in the 3'-5' direction. Helicase activity is coupled to the hydrolysis of ATP or dATP and to a lesser extent to CTP, dCTP, or UTP. It requires a divalent cation (Mg2+...

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Bibliographic Details
Published in:The Journal of biological chemistry 1993-01, Vol.268 (2), p.1220-1225
Main Authors: BOEHMER, P. E, DODSON, M. S, LEHMAN, I. R
Format: Article
Language:English
Subjects:
Base Composition
Base Sequence
Biological and medical sciences
Cations, Divalent
DNA Helicases - genetics
DNA Helicases - metabolism
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Fundamental and applied biological sciences. Psychology
Genes, Viral
herpes simplex virus 1
Kinetics
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Nucleotides - pharmacology
Oligodeoxyribonucleotides
Replication
Simplexvirus - enzymology
Simplexvirus - genetics
Substrate Specificity
Viral Proteins - genetics
Viral Proteins - metabolism
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Summary:The herpes simplex virus type 1 (HSV-1) origin binding protein, the product of the UL9 gene, catalyzes the unwinding of DNA in the 3'-5' direction. Helicase activity is coupled to the hydrolysis of ATP or dATP and to a lesser extent to CTP, dCTP, or UTP. It requires a divalent cation (Mg2+ > Mn2+ > Ca2+) with an optimum at 2.5 mM MgCl2. Activity is optimal at high pH (8.5-9.5) and high temperature (45 degrees C) and is inhibited at ionic strengths > 50 mM NaCl. The helicase activity is specifically stimulated by the HSV-1-encoded single-stranded DNA-binding protein, ICP8, which increases both the rate and extent of helicase activity. Helicase action appears to be stoichiometric, requiring a DNA-dependent assembly of a multimeric UL9 protein complex. Under optimal conditions, the rate of DNA unwinding is approximately 75 base pairs/min.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)54063-1