Early induction of Na +-dependent uridine uptake in the regenerating rat liver

Na +-dependent uridine transport into liver plasma membrane vesicles from partially hepatectomized and sham-operated rats was studied. Preparations purified 6 h after 70% hepatectomy exhibited an increased V max of uridine uptake (3.7 vs. 1.4 pmol/mg prot/3 s) without any change in Km (6μM). Incubat...

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Bibliographic Details
Published in:FEBS letters 1993-01, Vol.316 (1), p.85-88
Main Authors: Ruiz-Montasell, Bonaventura, Martinez-Mas, Joan Vicenç, Enrich, Carlos, Casado, F.Javier, Felipe, Antonio, Pastor-Anglada, Marçal
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biological Transport - drug effects
Cell Membrane - drug effects
Cell Membrane - metabolism
Fundamental and applied biological sciences. Psychology
Kinetics
Liver - drug effects
Liver - metabolism
Liver Regeneration
Liver. Bile. Biliary tracts
Monensin - pharmacology
Nucleoside transport
Rat liver
Rats
Rats, Wistar
Sodium - metabolism
Uridine - metabolism
Vertebrates: digestive system
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Summary:Na +-dependent uridine transport into liver plasma membrane vesicles from partially hepatectomized and sham-operated rats was studied. Preparations purified 6 h after 70% hepatectomy exhibited an increased V max of uridine uptake (3.7 vs. 1.4 pmol/mg prot/3 s) without any change in Km (6μM). Incubation of the vesicles in the presence of monensin decreased uridine uptake although the differences between both experimental groups remained identical. It is concluded that uridine transport is induced early after partial hepatectomy by a mechanism which does not involve changes in the transmembrane Na + gradient. This is the first evidence in favor of modulation of nucleoside transport into liver cells.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(93)81741-H