Identification of genes regulated by ammonium availability in the roots of maritime pine trees

• Published in: Amino acids 2010-10, Vol.39 (4), p.991-1001
• Main Authors: Canales, Javier, Flores-Monterrosso, Arantxa, Rueda-López, Marina, Avila, Concepción, Cánovas, Francisco M
• Format: Article
• Language: English
• Subjects: Amino acid biosynthesis; Amino Acids - biosynthesis; Ammonium nutrition; Ammonium-responsive genes; Analytical Chemistry; asparagine; Asparagine - biosynthesis; Biochemical Engineering; Biochemistry; Biomedical and Life Sciences; Coniferophyta; conifers; Gene expression; Gene Expression Regulation, Plant; Genes, Plant; glutamine; Glutamine - biosynthesis; Life Sciences; Neurobiology; Original Article; Pinus; Pinus - genetics; Pinus - metabolism; Plant Roots - genetics; Plant Roots - metabolism; Polymerase Chain Reaction; Protein Array Analysis; Proteomics; Quaternary Ammonium Compounds - metabolism; RNA, Plant - analysis; Signal Transduction; Stress, Physiological; Transcription, Genetic
• ISSN: 0939-4451; 1438-2199
• DOI: 10.1007/s00726-010-0483-9

Conifers have a preference for ammonium over nitrate as the main inorganic nitrogen source. However, it is unknown how changes in nitrogen nutrition may affect transcription profiles. In this study, microarray analysis and suppressive subtraction hybridization were used to identify differentially expressed genes in the roots of maritime pine exposed to changes in ammonium availability. A total of 225 unigenes that were differentially regulated by changes in ammonium nutrition were identified. Most of the unigenes were classified into seven functional categories by comparison with sequences deposited in the databases. A significant proportion of these genes were encoded for ammonium-regulated proteins of unknown functions. The differential expression of selected candidate genes was further validated in plants subjected to ammonium excess/deficiency. The transcript levels of representative genes were compared in maritime pine roots, 1, 15 and 35 days after nutritional treatments. Gene expression patterns suggest the existence of potential links between ammonium-responsive genes and genes involved in amino acid metabolism, particularly in asparagine biosynthesis and utilization. Functional analyses and exploration of the natural variability in maritime pine populations for a number of relevant genes are underway.