Oxazolone-induced over-expression of focal adhesion kinase in colonic epithelial cells of colitis mouse model

We examined the change of protein tyrosine kinases (PTKs) expression levels in colonic epithelial cells isolated from mice in which colitis was induced by oxazolone administration, using the monoclonal antibody YK34, which cross-reacts with a wide variety of PTKs. We identified focal adhesion kinase...

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Bibliographic Details
Published in:FEBS letters 2010-09, Vol.584 (18), p.3949-3954
Main Authors: Ma, Yanju, Semba, Shingo, Maemoto, Atsuo, Takeuchi, Masayuki, Kameshita, Isamu, Ishida, Atsuhiko, Kato, Sanae, Katoh, Tsuyoshi, Liu, Yunpeng, Taniguchi, Takanobu
Format: Article
Language:English
Subjects:
Animals
Colitis - chemically induced
Colitis - enzymology
Colitis - pathology
Colon - drug effects
Colon - enzymology
Crohn's disease
Disease Models, Animal
FAK
Focal adhesion kinase
Focal Adhesion Kinase 1 - biosynthesis
IBD
Inflammatory bowel disease
Intestinal Mucosa - drug effects
Intestinal Mucosa - enzymology
Intestinal Mucosa - pathology
Male
Mice
Mice, Inbred BALB C
Oxazolone - pharmacology
Protein tyrosine kinase
PTK
Ulcerative colitis
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Summary:We examined the change of protein tyrosine kinases (PTKs) expression levels in colonic epithelial cells isolated from mice in which colitis was induced by oxazolone administration, using the monoclonal antibody YK34, which cross-reacts with a wide variety of PTKs. We identified focal adhesion kinase (FAK) and found the expression level increased due to the induction of colitis. Furthermore, we found that there was a positive correlation between FAK expression and the severity of colitis. Also, FAK expression localized in the colonic epithelium but not in the lamina propria, implying FAK functions in epithelial cells during colitis formation and/or wound repairing.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2010.07.054