Synthesis and evaluation of 99mTc- N-sulfanilamide ferrocene carboxamide as bacterial infections detector

A technetium-99m-labeled derivative from sulfanilamide, further referred to as 99mTc-N-SFC, targeting infections in experimental animals, has been synthesized. The biological features of this radioactive agent have also been studied. The N-sulfanilamide ferrocene carboxamide (N-SFC) was chemically s...

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Bibliographic Details
Published in:Nuclear medicine and biology 2010-10, Vol.37 (7), p.821-829
Main Authors: Essouissi, Imen, Ghali, Wafa, Saied, Nadia Malek, Saidi, Mouldi
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Contrast media. Radiopharmaceuticals
Ferrous Compounds - chemical synthesis
Ferrous Compounds - pharmacokinetics
Infectious imaging
Medical sciences
Mice
Organotechnetium Compounds - chemical synthesis
Organotechnetium Compounds - pharmacokinetics
Pharmacology. Drug treatments
Radiolabeling
Radionuclide Imaging
Radiopharmaceuticals - chemical synthesis
Radiopharmaceuticals - pharmacokinetics
Staphylococcal Infections - diagnostic imaging
Staphylococcal Infections - microbiology
Staphylococcus aureus
Staphylococcus aureus - physiology
Sulfanilamide
Tissue Distribution
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Summary:A technetium-99m-labeled derivative from sulfanilamide, further referred to as 99mTc-N-SFC, targeting infections in experimental animals, has been synthesized. The biological features of this radioactive agent have also been studied. The N-sulfanilamide ferrocene carboxamide (N-SFC) was chemically synthesized and then labeled with technetium-99m. It has been confirmed through this work that it is stable and obtained with radiolabelling yield (>87%). Radiochemical analyses of 99mTc-N-SFC revealed that the molecule was labeled rapidly (within 2 min) and effectively with little free pertechnetate in the preparations containing purified compound. Furthermore, in vitro investigations were conducted and the label's stability in serum was observed up to 24 h of testing. Uptake of the tracer with live and heat/killed bacteria was compared in physiological conditions and was about 69% and 61.9% for the Escherichia coli and Staphylococcus aureus strains, respectively. We concluded that synthesis and labeling of Sulfanilamide derivative with 99m-Tc by this method is rapid, efficient and safe. Biodistribution studies demonstrated that our radiolabeled compound is accumulated rapidly and significantly ( P
ISSN:0969-8051
1872-9614
DOI:10.1016/j.nucmedbio.2010.04.139