Proliferation and differentiation of human adipocyte precursor cells: Differences between the preperitoneal and subcutaneous compartments

Human adipocyte precursor cells (APC) have been characterized in their proliferation and differentiation potential from subcutaneous, omental, and mesenteric depots, mostly from morbidly obese patients. Cells from the preperitoneal adipose compartment have not been characterized yet, least of all wh...

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Bibliographic Details
Published in:Journal of cellular biochemistry 2010-10, Vol.111 (3), p.659-664
Main Authors: Fernández, M., Acuña, M.J., Reyes, M., Olivares, D., Hirsch, S., Bunout, D., de la Maza, M.P.
Format: Article
Language:English
Subjects:
Adipocytes - cytology
adipose
Adipose Tissue - cytology
Case-Control Studies
Cell Differentiation
Cell Proliferation
differentiation
fat
human
Humans
Male
Obesity
Omentum
Peritoneal Cavity
preadipocyte
precursor
proliferation
Stem Cells
Subcutaneous Tissue
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Summary:Human adipocyte precursor cells (APC) have been characterized in their proliferation and differentiation potential from subcutaneous, omental, and mesenteric depots, mostly from morbidly obese patients. Cells from the preperitoneal adipose compartment have not been characterized yet, least of all when obtained from normal weight subjects. The aim was to compare proliferation and differentiation of subcutaneous (SC) and preperitoneal (PP) APC derived from adipose tissue in healthy subjects with different body mass. SC and PP adipose tissue was obtained during surgery of inguinal hernias in five healthy non‐obese subjects and three obese otherwise healthy men. APC, obtained by collagenase digestion, were cultured. Proliferation was assayed by cell counting and differentiation by oil red O staining and flow cytometry using Nile Red staining. Proliferation of SC was higher than PP APC. Such differences between both compartments were even higher in APC obtained from obese patients. Conversely PP APC differentiated earlier in vitro compared with SC cells. These results agree with published data on fat cell proliferation. However regarding differentiation, our data show that APC from deeper depots (in this case PP) differentiate earlier than subcutaneous APC. This is different to previous studies performed in mesenteric or omental adipose tissue. J. Cell. Biochem. 111: 659–664, 2010. © 2010 Wiley‐Liss, Inc.
ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.22753