Purification and immunolocalization on an annexin-like protein in pea seedlings

• Published in: Planta 1992, Vol.187 (1), p.1-9
• Main Authors: Clark, G.B. (Texas Univ., Austin (USA): Dept. of Botany), Dauwalder, M, Roux, S.J
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Analysemethode; Annexins; Annexins - analysis; Annexins - isolation & purification; ANTIGEN ANTIBODY REACTIONS; Biological and medical sciences; Calcium; Calcium - physiology; Cell biochemistry; Cell Membrane - chemistry; Cell Membrane - physiology; Cell Membrane - ultrastructure; Cell physiology; Epidermal cells; Erbse; Exocytosis - physiology; Fundamental and applied biological sciences. Psychology; Gels; Golgi Apparatus - metabolism; Golgi Apparatus - physiology; Golgi Apparatus - ultrastructure; Immunohistochemistry; Life Sciences (General); Microscopy, Electron; Molecular Sequence Data; Molecular Weight; Peas; PISUM SATIVUM; Pisum sativum - chemistry; Pisum sativum - cytology; Pisum sativum - physiology; Plant physiology and development; Plant Proteins - analysis; Plant Proteins - isolation & purification; Plant Root Cap - cytology; Plant Root Cap - ultrastructure; PLUMULA; PLUMULE; polyklonale Antikoerper; Protein; PROTEINAS; PROTEINE; PROTEINS; REACCION ANTIGENO-ANTICUERPO; REACTION ANTIGENE ANTICORPS; Root cap; Saemling; Secretion; Sieve elements; Xylem
• ISSN: 0032-0935; 1432-2048
• DOI: 10.1007/BF00201617

As part of a study to identify potential targets of calcium action in plant cells, a 35-kDa, annexin-like protein was purified from pea (Pisum sativum L.) plumules by a method used to purify animal annexins. This protein, called p35, binds to a phosphatidylserine affinity column in a calcium-dependent manner and binds 45Ca2+ in a dot-blot assay. Preliminary sequence data confirm a relationship for p35 with the annexin family of proteins. Polyclonal antibodies have been raised which recognize p35 in Western and dot blots. Immunofluorescence and immunogold techniques were used to study the distribution and subcellular localization of p35 in pea plumules and roots. The highest levels of immunostain were found in young developing vascular cells producing wall thickenings and in peripheral root-cap cells releasing slime. This localization in cells which are actively involved in secretion is of interest because one function suggested for the animal annexins is involvement in the mediation of exocytosis.