Antibody response to the M2 protein of influenza A virus expressed in insect cells

1 Influenza Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, 1600 Clifton Road, Centers for Disease Control, Atlanta, Georgia 30333, U.S.A. 2 Institute of Microbiology, Academy of Sciences, Riga, Latvia and 3 Institute for Virology, Philipps University, Ma...

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Bibliographic Details
Published in:Journal of general virology 1993-01, Vol.74 (1), p.143-146
Main Authors: Black, Renee A, Rota, Paul A, Gorodkova, Natalia, Klenk, Hans-Dieter, Kendal, Alan P
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
Antibodies, Viral - blood
baculovirus
Base Sequence
Biological and medical sciences
Blotting, Western
Ferrets
Fundamental and applied biological sciences. Psychology
Humans
Immunoenzyme Techniques
influenza A virus
Influenza A virus - immunology
Microbiology
Molecular Sequence Data
Moths
Recombinant Proteins - immunology
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
Spodoptera frugiperda
Viral Matrix Proteins - immunology
Virology
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Summary:1 Influenza Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, 1600 Clifton Road, Centers for Disease Control, Atlanta, Georgia 30333, U.S.A. 2 Institute of Microbiology, Academy of Sciences, Riga, Latvia and 3 Institute for Virology, Philipps University, Marburg, Germany A recombinant baculovirus expressing the M2 protein from influenza A/Ann Arbor/6/60 (H2N2) virus (AA60 virus) was constructed. The expressed M2 protein was recognized by a monoclonal antibody specific for the M2 protein and comigrated with the M2 protein from cells infected with AA60 virus on SDS-polyacrylamide gels. Immunofluorescence studies indicated that the expressed M2 protein was present on the surface of Spodoptera frugiperda (Sf9) cells infected with the recombinant baculovirus. Immunoassays using the expressed M2 protein were able to detect antibodies to the M2 protein in serum samples from humans and ferrets infected with influenza A viruses. Received 29 June 1992; accepted 18 September 1992.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-74-1-143