The caprine MHC contains DYA genes

Investigation of such associations requires definition of the caprine MHC class II region. In order to detect new genes in this region, we have used a genomic probe for a recently described bovine class II gene, Bota-DYA to examine the goat genome by pulsed field gel electrophoresis (PFGE). Goat DNA...

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Bibliographic Details
Published in:Immunogenetics (New York) 1993, Vol.37 (4), p.292-295
Main Authors: MANN, A. J, ABRAHAM, L. J, CAMERON, P. U, ROBINSON, W, GIPHART, M. J, DAWKINS, R. L
Format: Article
Language:English
Subjects:
amino acid sequence
Animals
Base Sequence
Biological and medical sciences
Cattle
cDNA
DNA
DYA loci
Electrophoresis, Gel, Pulsed-Field
Fundamental and applied biological sciences. Psychology
genes
Genes, MHC Class II
Genes. Genome
goats
Goats - genetics
Goats - immunology
major histocompatibility complex
Major Histocompatibility Complex - genetics
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
nucleotide sequence
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
predictions
Sequence Homology, Nucleic Acid
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Summary:Investigation of such associations requires definition of the caprine MHC class II region. In order to detect new genes in this region, we have used a genomic probe for a recently described bovine class II gene, Bota-DYA to examine the goat genome by pulsed field gel electrophoresis (PFGE). Goat DNA contained in agarose plugs was digested with a variety of rare cutting restriction enzymes and analyzed by PFGE. Southern hybridization analysis and probing sequentially with a Bota-DYA exon 3 fragment (347 base pairs (bp); van der Poel et al. 1990) and DRB (786 bp; Long et al. 1983) revealed that both DY and DR-hybridizing sequences are present on a 250 kilobase (kb) Not I fragment in all animals tested. One sample showed an additional 340 kb Not I fragment with both probes. Although partial digestion cannot be excluded, the detection of an additional fragment hybridizing to both DR and DY supports previous data that indicate the structure of the class II region may vary in different goat MHC haplotypes.
ISSN:0093-7711
1432-1211
DOI:10.1007/BF00187457