HLA-B51 transgenic mice as recipients for production of polymorphic HLA-A, B-specific antibodies

In the present study, we attempted to produce polymorphic antibodies for HLA-A, B, And C antigens in HLA-B51 TGM. TGM were produced by injecting the 6.4 kilobase (Kb) Eco RI fragment of the HLA-B51 (B super(5)101) gene into C3H/He one-cell embryos. Surviving embryos were reimplanted into the oviduct...

Full description

Saved in:
Bibliographic Details
Published in:Immunogenetics (New York) 1993, Vol.37 (2), p.139-142
Main Authors: Karaki, S, Kariyone, A, Kato, N, Kano, K, Iwakura, Y, Takiguchi, M
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - biosynthesis
Antibodies, Monoclonal - immunology
Cross Reactions
Flow Cytometry
HLA-A Antigens - immunology
HLA-B Antigens - genetics
HLA-B Antigens - immunology
HLA-B51 Antigen
HLA-C Antigens - immunology
Isoantibodies - biosynthesis
Isoantibodies - immunology
Mice
Mice, Inbred C3H
Mice, Inbred ICR
Mice, Transgenic
Polymorphism, Genetic
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In the present study, we attempted to produce polymorphic antibodies for HLA-A, B, And C antigens in HLA-B51 TGM. TGM were produced by injecting the 6.4 kilobase (Kb) Eco RI fragment of the HLA-B51 (B super(5)101) gene into C3H/He one-cell embryos. Surviving embryos were reimplanted into the oviducts of pseudopregnant ICR female mice. The offspring mice were tested for integration of the transgene by dot-blot hybridization of DNA derived from the tail with a 340 base pairs (bp) Beg II-Xba I fragment containing the exons 6 and 7 of the HLA-B51 gene. The surface expression of HLA-B51 antigen was detected on peripheral lymphocyte blood cells (BPL), thymocytes and spleen cells of the HLA-B51 TGM by flow cytometry using W6/32 HLA class I-specific mAb.
ISSN:0093-7711
1432-1211
DOI:10.1007/bf00216838