Increase in Ornithine Decarboxylase Activity Caused by Hepatocyte Growth Factor in Primary Cultured Adult Rat Hepatocytes

The effect of hepatocyte growth factor on ornithine decarboxylase activity was studied in primary cultured adult rat hepatocytes. Ornithine decarboxylase activity was increased 3 hr after the addition of hepatocyte growth factor and remained at a high level until 12 hr; thereafter it decreased, and...

Full description

Saved in:
Bibliographic Details
Published in:Hepatology (Baltimore, Md.) Md.), 1993-01, Vol.17 (1), p.99-102
Main Authors: Higaki, Ikko, Matsui‐yuasa, Isao, Terakura, Masanobu, Otani, Shuzo, Kinoshita, Hiroaki
Format: Article
Language:English
Subjects:
Actins - genetics
Animals
Biological and medical sciences
Cells, Cultured
Cycloheximide - pharmacology
Dose-Response Relationship, Drug
Epidermal Growth Factor - pharmacology
Fundamental and applied biological sciences. Psychology
Hepatocyte Growth Factor - pharmacology
Insulin - pharmacology
Liver - cytology
Liver - drug effects
Liver - enzymology
Liver. Bile. Biliary tracts
Ornithine Decarboxylase - genetics
Ornithine Decarboxylase - metabolism
Rats
RNA, Messenger - analysis
Time Factors
Vertebrates: digestive system
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The effect of hepatocyte growth factor on ornithine decarboxylase activity was studied in primary cultured adult rat hepatocytes. Ornithine decarboxylase activity was increased 3 hr after the addition of hepatocyte growth factor and remained at a high level until 12 hr; thereafter it decreased, and it returned to the control level by 24 hr. Enzyme activity began to increase with 1 ng/ml hepatocyte growth factor and reached its maximum with 5 ng/ml hepatocyte growth factor. When insulin or epidermal growth factor was added with hepatocyte growth factor, enzyme activity was further stimulated. The level of ornithine decarboxylase messenger RNA did not increase with addition of hepatocyte growth factor. The half‐time of ornithine decarboxylase activity was prolonged about twofold by hepatocyte growth factor treatment. These results suggest that hepatocyte growth factor treatment of cells enhanced ornithine decarboxylase activity posttranslationally (HEPATOLOGY 1993;17:99–102.)
ISSN:0270-9139
1527-3350
DOI:10.1002/hep.1840170118