Regulation of herpes simplex virus type 1 gene expression in nonpermissive murine resident peritoneal macrophages

The restriction of herpes simplex virus type 1 (HSV‐1) gene expression in nonpermissive murine resident peritoneal macrophages (ResPMO) was analyzed at the level of transcription. Using nuclear run‐on assays, we established that transcription of a representative gene from each HSV‐1 kinetic class wa...

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Bibliographic Details
Published in:Journal of leukocyte biology 1993-01, Vol.53 (1), p.61-65
Main Authors: Wu, Linxian, Morahan, Page S., Leary, Kathryn
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Nucleus - physiology
Cells, Cultured
Chloramphenicol O-Acetyltransferase - genetics
Chloramphenicol O-Acetyltransferase - metabolism
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Viral
Genes, Viral
Genetics
herpes simplex virus
herpesvirus
Kinetics
Macrophages - microbiology
Macrophages - physiology
Mice
Mice, Inbred Strains
Microbiology
mononuclear phagocyte
nonpermissivity
Plasmids
Recombinant Fusion Proteins - metabolism
Simplexvirus - genetics
Transcription, Genetic
Transfection
Vero Cells
Virology
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Summary:The restriction of herpes simplex virus type 1 (HSV‐1) gene expression in nonpermissive murine resident peritoneal macrophages (ResPMO) was analyzed at the level of transcription. Using nuclear run‐on assays, we established that transcription of a representative gene from each HSV‐1 kinetic class was initiated in both ResPMO and permissive Vero cells. Using transient expression assays, we observed that the promoters of two early genes were activated by the immediate‐early protein ICP4 in ResPMO. However, the level of trans‐ activation of these promoters by another immediate‐early protein, ICPO, was markedly reduced in ResPMO. Furthermore, the synergistic trans‐activation normally observed between ICPs 4 and O in Vero cells was noticeably absent in ResPMO. The data indicate that the block in viral gene expression in ResPMO occurs after initiation of viral gene transcription and involves dysfunction of a viral immediate‐early regulatory protein, ICP0.
ISSN:0741-5400
1938-3673
DOI:10.1002/jlb.53.1.61