Map of seven polymorphic markers on rat chromosome 14: linkage conservation with human chromosome 4

Seven polymorphic markers identified by polymerase chain reaction (PCR) amplification, including markers for six genes--DRD1L (dopamine receptor, D1-like-2), GLUKA (glucokinase), PF4 (platelet factor 4), ALB (albumin), AFP (alpha-fetoprotein), and BSP (bone sialoprotein)--and one anonymous locus (D1...

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Bibliographic Details
Published in:Mammalian genome 1993, Vol.4 (2), p.90-94
Main Authors: Remmers, E F, Goldmuntz, E A, Cash, J M, Zha, H, Crofford, L J, Misiewicz-Poltorak, B, Mathern, P, Wilder, R L
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Chromosome Mapping
Chromosomes, Human, Pair 4
Crosses, Genetic
DNA, Single-Stranded
Genetic Linkage
Genetic Markers
Humans
Molecular Sequence Data
Polymorphism, Genetic
Rats
Rats, Inbred F344
Rats, Inbred Lew
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Summary:Seven polymorphic markers identified by polymerase chain reaction (PCR) amplification, including markers for six genes--DRD1L (dopamine receptor, D1-like-2), GLUKA (glucokinase), PF4 (platelet factor 4), ALB (albumin), AFP (alpha-fetoprotein), and BSP (bone sialoprotein)--and one anonymous locus (D14N52), were mapped to a single 67-cM linkage group with F2 intercross progeny of F344/N and LEW/N inbred rat strains. Two of these markers, ALB and AFP, have previously been assigned to rat Chromosome (Chr) 14, allowing assignment of this entire linkage group. Five of the markers--DRD1L, PF4, ALB, AFP, and BSP--have been physically mapped to a large region of human Chr 4 encompassing the p arm and the q arm to band q28. Homologs of two of the markers, ALB and AFP, have been mapped to Chr 5 in the mouse. Comparison of human Chr 4 with the homologous regions on Chr 14 of the rat and Chr 5 of the mouse indicated that linkage conservation with human Chr 4 extends over a greater region in the rat than in the mouse. The markers described here were found to be highly polymorphic in twelve inbred strains (F344/N, LEW/N, ACI/N, BUF/N, BN/SsN, LOU/MN, MNR/N, MR/N, SHR/N, WBB1/N, WBB2/N, and WKY/N). These polymorphic markers should be useful in genetic linkage studies of important phenotypes in rats.
ISSN:0938-8990
1432-1777
DOI:10.1007/BF00290432