The mouse Eb meiotic recombination hotspot contains a tissue-specific transcriptional enhancer

A meiotic recombination hotspot exists within the second intron of the mouse major histocompatibility complex (MHC) gene, Eb. In the present study, a small fragment from the intron which contains two potential transcriptional regulatory elements was cloned into an expression vector and its effect on...

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Bibliographic Details
Published in:Immunogenetics (New York) 1993, Vol.37 (5), p.331-336
Main Authors: XIAOBING LING, SHENKAR, R, SAKAI, D, ARNHEIM, N
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Cell Line
Enhancer Elements, Genetic - genetics
Fibroblasts
Fundamental and applied biological sciences. Psychology
Genes, MHC Class II - genetics
Genes. Genome
Meiosis - genetics
Mice
Mice, Inbred C3H
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Plasmids - genetics
Recombination, Genetic - genetics
RNA, Messenger - genetics
Transfection
Tumor Cells, Cultured
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Summary:A meiotic recombination hotspot exists within the second intron of the mouse major histocompatibility complex (MHC) gene, Eb. In the present study, a small fragment from the intron which contains two potential transcriptional regulatory elements was cloned into an expression vector and its effect on transcription was tested. This fragment was found to contain tissue-specific transcriptional enhancer activity. An octamer-like sequence and a B motif may contribute to this enhancer activity. Similar regulatory sequences with the same orientation and distance from one another are found in another mouse MHC recombination hotspot.
ISSN:0093-7711
1432-1211
DOI:10.1007/bf00216797