The identification of Y chromosome-linked markers with random sequence oligonucleotide primers

• Published in: Mammalian genome 1993-01, Vol.4 (2), p.109-112
• Main Authors: Wardell, B B, Sudweeks, J D, Meeker, N D, Estes, S S, Woodward, S R, Teuscher, C
• Format: Article
• Language: English
• Subjects: Animals; Base Sequence; DNA, Single-Stranded; Female; Genetic Linkage; Genetic Markers; Male; Mice; Mice, Inbred Strains; Molecular Sequence Data; Polymerase Chain Reaction - methods; Species Specificity; Y Chromosome
• ISSN: 0938-8990; 1432-1777
• DOI: 10.1007/BF00290435

The polymerase chain reaction (PCR)-based technique of random amplification of polymorphic DNA (RAPD) is extremely useful for developing DNA-based markers. We previously identified a linkage group of eight unmapped RAPD markers that distinguish C57BL/6J and DBA/2J mice (Mammalian Genome 3: Woodward et al., 73-78, 1992). In this study, we report that all eight markers are Y Chromosome (Chr)-linked. One additional Y-linked RAPD was discovered serendipitously during the screening of a C3H/HeJ x (C3H/HeJ x SJL/J)F1 BC1 population. The segregation of all nine markers was analyzed with a panel of 14 independent inbred strains of male mice. The nine markers could be divided into three distinct groups: (1) DYByu2, DYByu5, DYByu6, and DYByu8 identify both the M.m. musculus and M.m. domesticus type Y Chr; (2) DYByu1, DYByu3, DYByu4, and DYByu7 are specific for the M.m. musculus type; and (3) DYByu9 is specific for the M.m. domesticus type. The results clearly indicate that the RAPD technique can be used to identify Y Chr-linked, DNA-based markers in mammalian species.