Selection of a control rat for conscious spontaneous hypertensive rats in studies of detrusor overactivity on the basis of measurement of intra-abdominal pressures

• Published in: Neurourology and urodynamics 2010-09, Vol.29 (7), p.1338-1343
• Main Authors: Jin, Long-Hu, Andersson, Karl-Erik, Kwon, Yong-Hyun, Yoon, Sang-Min, Lee, Tack
• Format: Article
• Language: English
• Subjects: Abdominal Cavity; Animals; Consciousness; cystometry; detrusor; Disease Models, Animal; Hypertension - physiopathology; Male; overactivity; Pressure; Rats; Rats, Inbred SHR; Rats, Inbred WKY; Rats, Sprague-Dawley; Rats, Wistar; Reproducibility of Results; Species Specificity; spontaneously hypertensive rat; urinary bladder; Urinary Bladder - physiopathology; Urinary Bladder, Overactive - physiopathology; Urination; Urodynamics; Wistar-Kyoto rat
• ISSN: 0733-2467; 1520-6777
• DOI: 10.1002/nau.20873

Aims We investigated which animal model is appropriate as a control for the spontaneous hypertensive rat (SHR) in studies of detrusor overactivity (DO). Methods Age‐matched SHRs (n = 10), Wistar‐Kyoto (WKY) rats (n = 9), Wistar‐ST (Wistar) rats (n = 10), and Sprague–Dawley (SD) rats (n = 10) were studied. A balloon‐fitted catheter was positioned in the abdominal cavity to record intra‐abdominal pressure (IAP). Cystometric pressure‐ and volume‐related parameters and DO‐related ones during the filling phase were investigated. Of the intravesical pressure (IVP) rises above 2 cmH2O, DO was defined as a rise in IVP without a simultaneous change in IAP and was counted during the filling phase. Results Compared with SHRs, the WKY rats did not show a significant difference in pressure or volume parameters, but Wistar rats and SD rats showed a significant increase in volume parameters, such as bladder capacity, micturition volume, and micturition interval. Typical DO was seen in SHRs and WKY rats during the filling phase, but not in Wistar and SD rats. Furthermore, the frequency and increased amplitude of the DO were similar in WKY rats and SHRs. Conclusion Although WKY rats are an appropriate control for SHRs in studies of hypertension, they may be inappropriate as controls in studies of DO, because they have inherent DO of similar frequency and amplitude as found in SHRs. Neurourol. Urodynam. 29:1338–1343, 2010. © 2010 Wiley‐Liss, Inc.