DNA sequence and transcriptional analysis of the tblA gene required for tabtoxin biosynthesis by Pseudomonas syringae

The tblA gene of Pseudomonas syringae is required for tabtoxin biosynthesis and is under the control of a regulatory gene, lemA. We have determined the nucleotide sequence of the tblA gene and identified the 5' end of the tblA gene transcript. The sequence of the tblA gene was identical to that...

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Published in:Applied and Environmental Microbiology 1993-02, Vol.59 (2), p.458-466
Main Authors: BARTA, T. M, KINSCHERF, T. G, UCHYTIL, T. F, WILLIS, D. K
Format: Article
Language:English
Subjects:
arn mensajero
arn messager
Base Sequence
Biological and medical sciences
Deoxyribonucleic acid
Dipeptides - biosynthesis
Dipeptides - genetics
DNA
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
Flowers & plants
Fundamental and applied biological sciences. Psychology
gene
genes
Genes. Genome
Genetics
messenger rna
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
nucleotide sequence
Open Reading Frames
Pseudomonas - chemistry
Pseudomonas - genetics
Pseudomonas - growth & development
Pseudomonas - metabolism
Pseudomonas syringae
RNA, Bacterial - genetics
secuencia nucleica
Sequence Analysis, DNA
sequence nucleique
toxinas
toxine
toxins
Transcription, Genetic - genetics
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KINSCHERF, T. G
UCHYTIL, T. F
WILLIS, D. K
description The tblA gene of Pseudomonas syringae is required for tabtoxin biosynthesis and is under the control of a regulatory gene, lemA. We have determined the nucleotide sequence of the tblA gene and identified the 5' end of the tblA gene transcript. The sequence of the tblA gene was identical to that of the recently reported open reading frame 1 gene of the tabA region of the BR2 chromosome. The open reading frame of the tblA gene potentially encodes a protein of 231 amino acids. mRNA from the tblA gene was detected at all phases of cells grown in minimal medium. This result is correlated with the constitutive production of tabtoxinine-beta-lactam (the biologically active part of the toxin) by P. syringae BR2R in minimal medium, as quantitated by a phenylisothiocyanate derivatization method.
doi_str_mv 10.1128/AEM.59.2.458-466.1993
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Genome</topic><topic>Genetics</topic><topic>messenger rna</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequence</topic><topic>Open Reading Frames</topic><topic>Pseudomonas - chemistry</topic><topic>Pseudomonas - genetics</topic><topic>Pseudomonas - growth &amp; development</topic><topic>Pseudomonas - metabolism</topic><topic>Pseudomonas syringae</topic><topic>RNA, Bacterial - genetics</topic><topic>secuencia nucleica</topic><topic>Sequence Analysis, DNA</topic><topic>sequence nucleique</topic><topic>toxinas</topic><topic>toxine</topic><topic>toxins</topic><topic>Transcription, Genetic - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BARTA, T. M</creatorcontrib><creatorcontrib>KINSCHERF, T. G</creatorcontrib><creatorcontrib>UCHYTIL, T. F</creatorcontrib><creatorcontrib>WILLIS, D. 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The sequence of the tblA gene was identical to that of the recently reported open reading frame 1 gene of the tabA region of the BR2 chromosome. The open reading frame of the tblA gene potentially encodes a protein of 231 amino acids. mRNA from the tblA gene was detected at all phases of cells grown in minimal medium. This result is correlated with the constitutive production of tabtoxinine-beta-lactam (the biologically active part of the toxin) by P. syringae BR2R in minimal medium, as quantitated by a phenylisothiocyanate derivatization method.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>7679566</pmid><doi>10.1128/AEM.59.2.458-466.1993</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record>
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source NCBI_PubMed Central（免费）; American Society for Microbiology (ASM) Journals
subjects arn mensajero
arn messager
Base Sequence
Biological and medical sciences
Deoxyribonucleic acid
Dipeptides - biosynthesis
Dipeptides - genetics
DNA
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
Flowers & plants
Fundamental and applied biological sciences. Psychology
gene
genes
Genes. Genome
Genetics
messenger rna
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
nucleotide sequence
Open Reading Frames
Pseudomonas - chemistry
Pseudomonas - genetics
Pseudomonas - growth & development
Pseudomonas - metabolism
Pseudomonas syringae
RNA, Bacterial - genetics
secuencia nucleica
Sequence Analysis, DNA
sequence nucleique
toxinas
toxine
toxins
Transcription, Genetic - genetics
title DNA sequence and transcriptional analysis of the tblA gene required for tabtoxin biosynthesis by Pseudomonas syringae
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