Prothymosin α gene in humans : organization of its promoter region and localization to chromosome 2

• Published in: Human genetics 1993-02, Vol.90 (6), p.629-634
• Main Authors: SZABO, P, PANNEERSELVAM, C, CLINTON, M, FRANGOU-LAZARIDIS, M, WEKSLER, D, WHITTINGTON, E, MACERA, M. J, GRZESCHIK, K.-H, SELVAKUMAR, A, HORECKER, B. L
• Format: Article
• Language: English
• Subjects: Base Sequence; Biological and medical sciences; chromosome 2; Chromosomes, Human, Pair 2; Classical genetics, quantitative genetics, hybrids; Cloning, Molecular; Deoxyribonuclease BamHI; Fundamental and applied biological sciences. Psychology; genes; Genetics of eukaryotes. Biological and molecular evolution; Human; Humans; localization; man; Molecular Sequence Data; Nucleic Acid Hybridization; nucleotide sequence; Polymorphism, Genetic; Promoter Regions, Genetic; promoters; Protein Precursors - genetics; prothymosin; Repetitive Sequences, Nucleic Acid; Sequence Analysis, DNA; Thymosin - analogs & derivatives; Thymosin - genetics
• ISSN: 0340-6717; 1432-1203
• DOI: 10.1007/BF00202480

A genomic clone encoding prothymosin alpha (gene symbol: PTMA), a nuclear-targeted protein associated with cell proliferation, was isolated and the 5'-regulatory region subcloned and sequenced. Because of previously reported discrepancies between several cDNA clones and a genomic clone for prothymosin alpha, we determined the sequence of the first exon and of a 1.7-kb region 5' to the first exon. The sequence of the genomic clone reported here corresponds to the published cDNA sequences, suggesting that the previously noted discrepancies may be due to genetic polymorphism in this region. In addition, our sequence data extend the known 5'-upstream sequence by an additional 1.5 kb allowing the identification of numerous, potential cis-acting regulatory sites. This 5'-flanking cloned probe permitted us to localize the prothymosin gene to chromosome 2 in humans.