Molecular Analysis of Multiply Recurrent Meningitis Due to Escherichia coli K1 in an Infant

Bacterial DNA polymorphism was used to document the occurrence ofthree separate episodes of meningitis caused by Escherichia coli K1 in an infant. The methods employed included determination of the restriction fragment length polymorphism of total DNA and of ribosomal DNA regions as well as DNA fing...

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Bibliographic Details
Published in:Clinical infectious diseases 1993-01, Vol.16 (1), p.82-85
Main Authors: Bingen, E., Caveé, H., Aujard, Y., Lambert-Zechovsky, N., Desjardins, P., Elion, J., Denamur, E.
Format: Article
Language:English
Subjects:
Bacterial meningitis
Clinical Infectious Disease Articles
DNA
DNA, Bacterial - analysis
Escherichia coli
Escherichia coli - classification
Escherichia coli - isolation & purification
Escherichia coli Infections - microbiology
Escherichia coli meningitis
Genetic polymorphism
Humans
Infant, Newborn
Infants
Male
Meningitis, Bacterial - microbiology
Polymerase Chain Reaction
Polymorphism, Genetic
Recurrence
Relapse
Restriction fragment length polymorphism
Ribosomal DNA
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Summary:Bacterial DNA polymorphism was used to document the occurrence ofthree separate episodes of meningitis caused by Escherichia coli K1 in an infant. The methods employed included determination of the restriction fragment length polymorphism of total DNA and of ribosomal DNA regions as well as DNA fingerprinting by the arbitrarily primed polymerase chain reaction. By these three genotypic approaches, the three isolates obtained from the infant's cerebrospinal fluid on days 9, 34, and 70, respectively, were found to share the same patterns, which were different from the patterns of control strains. Thus, these three episodes of E. coli K1 meningitis were due to a single strain. DNA-based typing techniques seem extremely promising as tools to be used in unraveling the complex mechanisms of recurrent meningitis.
ISSN:1058-4838
1537-6591
DOI:10.1093/clinids/16.1.82