The glycoprotein B homologue of human herpesvirus 6

• Published in: Journal of general virology 1993-03, Vol.74 (3), p.495-500
• Main Authors: Ellinger, K, Neipel, F, Foa-Tomasi, L, Campadelli-Fiume, G, Fleckenstein, B
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Antibodies, Monoclonal; Base Sequence; Biological and medical sciences; Chromosome Mapping; Fundamental and applied biological sciences. Psychology; Genetics; glycoprotein B; glycoprotein gp58 /116; herpesvirus (human) 6; Herpesvirus 6, Human - genetics; homology; Microbiology; Molecular Sequence Data; nucleotide sequence; Open Reading Frames - genetics; Protein Processing, Post-Translational - physiology; Viral Envelope Proteins - genetics; Viral Envelope Proteins - metabolism; Virology
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-74-3-495

1 Institut für klinische und molekulare Virologie, Universität Erlangen-Nürnberg, Loschgestrasse 7, D-8520 Erlangen, Germany and 2 Universita Degli Studi di Bologna, Sezione di Microbiologia e Virologia, Via San Giacomo no. 12, 40 126 Bologna, Italy The gene for the homologue of herpesvirus glycoprotein B (gB) has been identified in the genome of human herpesvirus 6 (HHV-6), strain U1102, and the nucleotide sequence was determined. The open reading frame encodes a protein of 830 amino acids (93.2K) with the characteristics of a transmembrane glycoprotein and close similarity to the gp58/116 complex of human cytomegalovirus (HCMV). Monoclonal antibodies 2D10 and 2B9 have been shown previously to react with an HHV-6 glycoprotein of apparent M r 112K, and its proteolytic cleavage products of M r 64K and 58K. We show that both monoclonal antibodies detect prokaryotically expressed carboxy-terminal fragments of the HHV-6 gB homologue. This indicates that the HHV-6 gB homologue is probably processed by proteolytic cleavage similar to its equivalents in HCMV and various other herpesviruses. Received 21 September 1992; accepted 10 November 1992.