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A mutant of herpes simplex virus type 1 in which the UL13 protein kinase gene is disrupted
1 Institute of Virology, University of Glasgow, Church Street, Glasgow G11 5JR and 2 Beatson Institute for Cancer Research, Wolfson Laboratory for Molecular Pathology, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, U.K. Gene UL13 of herpes simplex virus type 1 (HSV-1) has previously be...
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| Published in: | Journal of general virology 1993-03, Vol.74 (3), p.387-395 |
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| container_end_page | 395 |
| container_issue | 3 |
| container_start_page | 387 |
| container_title | Journal of general virology |
| container_volume | 74 |
| creator | Coulter, L. J Moss, H. W. M Lang, J McGeoch, D. J |
| description | 1 Institute of Virology, University of Glasgow, Church Street, Glasgow G11 5JR
and 2 Beatson Institute for Cancer Research, Wolfson Laboratory for Molecular Pathology, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, U.K.
Gene UL13 of herpes simplex virus type 1 (HSV-1) has previously been proposed to encode a protein kinase. An HSV-1 mutant with UL13 inactivated by insertion of the Escherichia coli lacZ gene was constructed. This UL13- lacZ mutant was found to grow to near wild-type (wt) titres in tissue culture. Comparison of silver-stained SDS-PAGE profiles of wt and UL13- lacZ virions demonstrated that the UL13 protein is a readily detectable component of wt virions, located in the tegument and probably equivalent to the previously described species VP18.8. Studies of in vitro phosphorylation with nuclear extracts of virus-infected cells and with detergent-treated virions showed that the UL13 protein is involved in phosphorylation of the tegument protein VP22. Extracts of cells engineered to express UL13, and infected with UL13- lacZ virus, were also capable of VP22 phosphorylation.
Members of the MRC Virology Unit.
Present address: Ohio State University, Department of Hematology and Oncology, 1248 James Cancer Hospital, 300 West Tenth Avenue, Columbus, Ohio 43210, U.S.A.
Received 3 July 1992;
accepted 16 October 1992. |
| doi_str_mv | 10.1099/0022-1317-74-3-387 |
| format | article |
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and 2 Beatson Institute for Cancer Research, Wolfson Laboratory for Molecular Pathology, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, U.K.
Gene UL13 of herpes simplex virus type 1 (HSV-1) has previously been proposed to encode a protein kinase. An HSV-1 mutant with UL13 inactivated by insertion of the Escherichia coli lacZ gene was constructed. This UL13- lacZ mutant was found to grow to near wild-type (wt) titres in tissue culture. Comparison of silver-stained SDS-PAGE profiles of wt and UL13- lacZ virions demonstrated that the UL13 protein is a readily detectable component of wt virions, located in the tegument and probably equivalent to the previously described species VP18.8. Studies of in vitro phosphorylation with nuclear extracts of virus-infected cells and with detergent-treated virions showed that the UL13 protein is involved in phosphorylation of the tegument protein VP22. Extracts of cells engineered to express UL13, and infected with UL13- lacZ virus, were also capable of VP22 phosphorylation.
Members of the MRC Virology Unit.
Present address: Ohio State University, Department of Hematology and Oncology, 1248 James Cancer Hospital, 300 West Tenth Avenue, Columbus, Ohio 43210, U.S.A.
Received 3 July 1992;
accepted 16 October 1992.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-74-3-387</identifier><identifier>PMID: 8383174</identifier><identifier>CODEN: JGVIAY</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Animals ; Biological and medical sciences ; Cell Line ; Chromosome Mapping ; Cricetinae ; Escherichia coli - genetics ; Fundamental and applied biological sciences. Psychology ; Genes, Viral - genetics ; Genes, Viral - physiology ; herpes simplex virus 1 ; Lac Operon ; Microbiology ; Mutation - genetics ; Mutation - physiology ; Phosphorylation ; Protein Kinases - genetics ; Protein Kinases - metabolism ; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains ; Simplexvirus - genetics ; Simplexvirus - isolation & purification ; Virion - chemistry ; Virology</subject><ispartof>Journal of general virology, 1993-03, Vol.74 (3), p.387-395</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-1eb3d705864d240f9c19727a5b5a66bcc136359b710acb0c77b58553900c3e2f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4696778$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8383174$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Coulter, L. J</creatorcontrib><creatorcontrib>Moss, H. W. M</creatorcontrib><creatorcontrib>Lang, J</creatorcontrib><creatorcontrib>McGeoch, D. J</creatorcontrib><title>A mutant of herpes simplex virus type 1 in which the UL13 protein kinase gene is disrupted</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>1 Institute of Virology, University of Glasgow, Church Street, Glasgow G11 5JR
and 2 Beatson Institute for Cancer Research, Wolfson Laboratory for Molecular Pathology, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, U.K.
Gene UL13 of herpes simplex virus type 1 (HSV-1) has previously been proposed to encode a protein kinase. An HSV-1 mutant with UL13 inactivated by insertion of the Escherichia coli lacZ gene was constructed. This UL13- lacZ mutant was found to grow to near wild-type (wt) titres in tissue culture. Comparison of silver-stained SDS-PAGE profiles of wt and UL13- lacZ virions demonstrated that the UL13 protein is a readily detectable component of wt virions, located in the tegument and probably equivalent to the previously described species VP18.8. Studies of in vitro phosphorylation with nuclear extracts of virus-infected cells and with detergent-treated virions showed that the UL13 protein is involved in phosphorylation of the tegument protein VP22. Extracts of cells engineered to express UL13, and infected with UL13- lacZ virus, were also capable of VP22 phosphorylation.
Members of the MRC Virology Unit.
Present address: Ohio State University, Department of Hematology and Oncology, 1248 James Cancer Hospital, 300 West Tenth Avenue, Columbus, Ohio 43210, U.S.A.
Received 3 July 1992;
accepted 16 October 1992.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Chromosome Mapping</subject><subject>Cricetinae</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Viral - genetics</subject><subject>Genes, Viral - physiology</subject><subject>herpes simplex virus 1</subject><subject>Lac Operon</subject><subject>Microbiology</subject><subject>Mutation - genetics</subject><subject>Mutation - physiology</subject><subject>Phosphorylation</subject><subject>Protein Kinases - genetics</subject><subject>Protein Kinases - metabolism</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>Simplexvirus - genetics</subject><subject>Simplexvirus - isolation & purification</subject><subject>Virion - chemistry</subject><subject>Virology</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><recordid>eNqFkctKxDAUhoMoOl5eQBCyEF1Vk-bWLmXwBgNunI2bkKanNtqbSevo25thhtlKFoHzf-fk8AWhc0puKMnzW0LSNKGMqkTxhCUsU3toRrkUSRrjfTTbAUfoOIQPQijnQh2iw4xlscpn6O0Ot9NouhH3Fa7BDxBwcO3QwA_-dn4KePwdAFPsOryqna3xWANeLijDg-9HiOVP15kA-B06wC7g0gU_DSOUp-igMk2As-19gpYP96_zp2Tx8vg8v1skljM-JhQKVioiMsnLlJMqtzRXqTKiEEbKwlrKJBN5oSgxtiBWqUJkQrCcEMsgrdgJutrMjQt9TRBG3bpgoWlMB_0UtBKS5jIT_4JU8ngEj2C6Aa3vQ_BQ6cG71vhfTYlem9drsXotViuumY7mY9PFdvpUtFDuWraqY365zU2wpqm86awLO4zLXCqVRex6g9XuvV45DzqKbV3cpHC9jl-ye_APUPSXOA</recordid><startdate>19930301</startdate><enddate>19930301</enddate><creator>Coulter, L. J</creator><creator>Moss, H. W. M</creator><creator>Lang, J</creator><creator>McGeoch, D. J</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19930301</creationdate><title>A mutant of herpes simplex virus type 1 in which the UL13 protein kinase gene is disrupted</title><author>Coulter, L. J ; Moss, H. W. M ; Lang, J ; McGeoch, D. J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-1eb3d705864d240f9c19727a5b5a66bcc136359b710acb0c77b58553900c3e2f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Chromosome Mapping</topic><topic>Cricetinae</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Viral - genetics</topic><topic>Genes, Viral - physiology</topic><topic>herpes simplex virus 1</topic><topic>Lac Operon</topic><topic>Microbiology</topic><topic>Mutation - genetics</topic><topic>Mutation - physiology</topic><topic>Phosphorylation</topic><topic>Protein Kinases - genetics</topic><topic>Protein Kinases - metabolism</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>Simplexvirus - genetics</topic><topic>Simplexvirus - isolation & purification</topic><topic>Virion - chemistry</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Coulter, L. J</creatorcontrib><creatorcontrib>Moss, H. W. M</creatorcontrib><creatorcontrib>Lang, J</creatorcontrib><creatorcontrib>McGeoch, D. J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Coulter, L. J</au><au>Moss, H. W. M</au><au>Lang, J</au><au>McGeoch, D. J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A mutant of herpes simplex virus type 1 in which the UL13 protein kinase gene is disrupted</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1993-03-01</date><risdate>1993</risdate><volume>74</volume><issue>3</issue><spage>387</spage><epage>395</epage><pages>387-395</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><coden>JGVIAY</coden><abstract>1 Institute of Virology, University of Glasgow, Church Street, Glasgow G11 5JR
and 2 Beatson Institute for Cancer Research, Wolfson Laboratory for Molecular Pathology, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, U.K.
Gene UL13 of herpes simplex virus type 1 (HSV-1) has previously been proposed to encode a protein kinase. An HSV-1 mutant with UL13 inactivated by insertion of the Escherichia coli lacZ gene was constructed. This UL13- lacZ mutant was found to grow to near wild-type (wt) titres in tissue culture. Comparison of silver-stained SDS-PAGE profiles of wt and UL13- lacZ virions demonstrated that the UL13 protein is a readily detectable component of wt virions, located in the tegument and probably equivalent to the previously described species VP18.8. Studies of in vitro phosphorylation with nuclear extracts of virus-infected cells and with detergent-treated virions showed that the UL13 protein is involved in phosphorylation of the tegument protein VP22. Extracts of cells engineered to express UL13, and infected with UL13- lacZ virus, were also capable of VP22 phosphorylation.
Members of the MRC Virology Unit.
Present address: Ohio State University, Department of Hematology and Oncology, 1248 James Cancer Hospital, 300 West Tenth Avenue, Columbus, Ohio 43210, U.S.A.
Received 3 July 1992;
accepted 16 October 1992.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>8383174</pmid><doi>10.1099/0022-1317-74-3-387</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of general virology, 1993-03, Vol.74 (3), p.387-395 |
| issn | 0022-1317 1465-2099 |
| language | eng |
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| source | Freely Accessible Journals |
| subjects | Animals Biological and medical sciences Cell Line Chromosome Mapping Cricetinae Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genes, Viral - genetics Genes, Viral - physiology herpes simplex virus 1 Lac Operon Microbiology Mutation - genetics Mutation - physiology Phosphorylation Protein Kinases - genetics Protein Kinases - metabolism Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Simplexvirus - genetics Simplexvirus - isolation & purification Virion - chemistry Virology |
| title | A mutant of herpes simplex virus type 1 in which the UL13 protein kinase gene is disrupted |
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