A functional map of the fruit-specific promoter of the tomato 2A11 gene

The 5' region of the fruit-specific tomato gene, 2A11, contains both positive and negative regulatory elements. We divided the 5' promoter region of the 2A11 gene into small fragments, ranging in size from 211 to 634 bp and used these short DNA fragments in in vitro protein-binding studies...

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Bibliographic Details
Published in:Plant molecular biology 1993-02, Vol.21 (4), p.625-640
Main Authors: VAN HAAREN, M. J. J, HOUCK, C. M
Format: Article
Language:English
Subjects:
Binding Sites
Biological and medical sciences
carte genetique
Cloning, Molecular
expresion genica
expression des genes
Fundamental and applied biological sciences. Psychology
gene
Gene Expression
Gene Expression Regulation
genes
Genes, Plant
genetic maps
In Vitro Techniques
Lycopersicon esculentum
mapas geneticos
Molecular and cellular biology
Molecular genetics
Nuclear Proteins - genetics
Plants, Genetically Modified - genetics
Promoter Regions, Genetic
Regulatory Sequences, Nucleic Acid
Restriction Mapping
RNA, Messenger - genetics
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Summary:The 5' region of the fruit-specific tomato gene, 2A11, contains both positive and negative regulatory elements. We divided the 5' promoter region of the 2A11 gene into small fragments, ranging in size from 211 to 634 bp and used these short DNA fragments in in vitro protein-binding studies. These studies revealed the presence of at least four fruit-specific and one leaf- and fruit-active protein-binding domains. These promoter fragments, as well as other overlapping fragments, were tested for their ability to enhance expression from a truncated heterologous promoter in transgenic plants. This analysis showed the presence of four fruit-specific and three general or leaf-active positive regulatory elements. Comparison of the results obtained with these two approaches allowed us to draw a functional map of the 2A11 promoter.
ISSN:0167-4412
1573-5028
DOI:10.1007/bf00014546