Down-regulation of human immunodeficiency virus type (HIV-1) production after stimulation of monocyte-derived macrophages infected with HIV-1

Macrophages infected with human immunodeficiency virus (HIV) can be stimulated as a result of secondary infections. The effect of stimulation of HIV-1-infected monocyte-derived macrophages on HIV-1 production by these cells was studied. Exposure of macrophages to phorbol 12-myristate 13-acetate or t...

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Bibliographic Details
Published in:The Journal of infectious diseases 1993-04, Vol.167 (4), p.810-817
Main Authors: Nottet, H S, de Graaf, L, Machiel de Vos, N, Bakker, L J, van Strijp, J A, Visser, M R, Verhoef, J
Format: Article
Language:English
Subjects:
Acetylcysteine - pharmacology
AIDS/HIV
Cell Survival
Cells, Cultured
Down-Regulation
HIV Core Protein p24 - analysis
HIV-1 - physiology
Humans
Luminescent Measurements
Macrophage Activation
Macrophages - microbiology
Macrophages - physiology
Mannitol - pharmacology
Oxidation-Reduction - drug effects
Phagocytosis
Superoxide Dismutase - pharmacology
Virus Replication - drug effects
Virus Replication - physiology
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Summary:Macrophages infected with human immunodeficiency virus (HIV) can be stimulated as a result of secondary infections. The effect of stimulation of HIV-1-infected monocyte-derived macrophages on HIV-1 production by these cells was studied. Exposure of macrophages to phorbol 12-myristate 13-acetate or to opsonized Escherichia coli, Staphylococcus aureus, or zymosan resulted in a decrease in HIV production. HIV production was inversely related to the degree of stimulation, measured as lucigenin-enhanced chemoluminescence. The production of reactive oxygen intermediates, however, did not seem to be the direct cause of the diminished HIV production, since oxygen-radical scavengers did not prevent the decrease in HIV production. Furthermore, oxygen-radical scavengers did not affect HIV production by nonstimulated macrophages. These results indicate that activation signals have an opposite effect and reactive oxygen intermediates have no effect on HIV production in macrophages compared with the effect described in T cells.
ISSN:0022-1899