Mouse Lymphoid Leukemias: Symbiotic Complexes of Neoplastic Lymphocytes and Their Microenvironments

Of 17 primary lymphoid leukemias of the mouse, 15 symbiotic cell lines were isolated by the explantation of leukemia tissues from which free leukemia cells had been mechanically removed. In vitro survival and growth of symbiotic leukemia cells depended on close association with the adherent cells fr...

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Bibliographic Details
Published in:JNCI : Journal of the National Cancer Institute 1981-04, Vol.66 (4), p.713-722
Main Authors: Hial, Hiroshi, Nishi, Yoshimi, Mlyazawa, Takahiko, Matsudalra, Yasue, Nishizuka, Yasuaki
Format: Article
Language:English
Subjects:
Animals
Cell Adhesion
Cell Line
Cell Membrane - ultrastructure
Cell Separation
Environment
Leukemia, Lymphoid - pathology
Leukemia, Lymphoid - ultrastructure
Lymphocytes - pathology
Mercaptoethanol - pharmacology
Mice
Microscopy, Electron, Scanning
Symbiosis - drug effects
Thymus Gland - cytology
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Summary:Of 17 primary lymphoid leukemias of the mouse, 15 symbiotic cell lines were isolated by the explantation of leukemia tissues from which free leukemia cells had been mechanically removed. In vitro survival and growth of symbiotic leukemia cells depended on close association with the adherent cells from the initial explants or other sources. Pseudo-em peripolesis was a remarkable morphologic manifestation of symbiosis common to all cell lines, Le., the leukemia cells were beneath the adherent cells in close contact. Cell interaction in symbiotic leukemias was studied with a representative symbiotic leukemia AKRL-3 and a cell line B6TE-A from normal thymic epithelium. Failure of the culture supernatant of the adherent cells to support the growth of leukemia cells indicated that the function of the adherent cells was mediated by close cell contact. During the culture, many symbiotic cell lines changed growth patterns and eventually grew independently. Consistent isolation of symbiotic c~1I lines from most primary leukemias, as well as consideration of the role of the thymus in leukemogenesis, may indicate that thEl lymphoid leukemias are basically symbiotic complexes of neoplastic lymphocytes and their microenvironments in their natural history. Similar Iymphoepithelial cell complexes were isolated recently from normal murine thymus.
ISSN:0027-8874
1460-2105
DOI:10.1093/jnci/66.4.713