highly selective and sensitive dopamine and uric acid biosensor fabricated with functionalized ordered mesoporous carbon and hydrophobic ionic liquid

• Published in: Analytical and bioanalytical chemistry 2010-03, Vol.396 (5), p.1755-1762
• Main Authors: Dong, Junping, Hu, Yanyan, Zhu, Shenmin, Xu, Jiaqiang, Xu, Yinjuan
• Format: Article
• Language: English
• Subjects: Analytical Chemistry; ascorbic acid; Ascorbic Acid - analysis; Biochemistry; Biological and medical sciences; Biosensing Techniques - methods; Biosensors; Biotechnology; Carbon; Carbon - chemistry; Carboxylic Acids - chemistry; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Detectors; Diffraction; Dopamine; Dopamine - analysis; Electrochemistry; Electrodes; Electron transport; Exact sciences and technology; Food Science; Fundamental and applied biological sciences. Psychology; General, instrumentation; Ionic liquid; Ionic liquids; Ionic Liquids - chemistry; Laboratory Medicine; Materials Testing; Mathematical analysis; Methods. Procedures. Technologies; Monitoring/Environmental Analysis; Ordered mesoporous carbon; Organic acids; Original Paper; Oxidation-Reduction; Particle Size; Phenols; Porosity; Surface Properties; Uric acid; Uric Acid - analysis; Various methods and equipments; X-rays
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-009-3423-3

An ordered mesoporous carbon material functionalized with carboxylic acid groups was synthesized. It was characterized by powder X-ray diffraction, transmission electron microscopy, Fourier transform IR spectroscopy and N₂ adsorption/desorption. Furthermore, this material was used to modify an electrode surface combined with a hydrophobic ionic liquid. The functionalized ordered mesoporous carbon/ionic liquid gel modified electrode shows excellent electrocatalytic performances for the oxidation of dopamine, uric acid and ascorbic acid. The presence of the ionic liquid promotes the electron transfer. Linear responses for dopamine and uric acid were obtained in the ranges of 0.1 to 500 μM and from 0.1 to 100 μM with detection limits of 4.1 and 2.5 nM (signal-to-noise ratio of 3), respectively, under optimum conditions. A quick and sensitive biosensor based on functionalized ordered mesoporous carbon and an ionic liquid has been developed for the first time for the detection of dopamine and uric acid in the presence of a large amount of ascorbic acid. [graphic removed]