In vitro growth of Babesia bovis in white-tailed deer (Odocoileus virginianus) erythrocytes

Babesia bovis cultured in bovine erythrocytes was passaged into white-tailed deer (Odocoileus virginianus) erythrocytes and medium containing either white-tailed deer serum or bovine serum. Deer erythrocytes supported the growth of the parasite only in the presence of bovine serum. Cryopreserved cul...

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Bibliographic Details
Published in:The Journal of parasitology 1993-04, Vol.79 (2), p.233-237
Main Authors: Holman, Patricia J., Waldrup, K. A., Droleskey, R. E., Corrier, D. E., Wagner, G. Gale
Format: Article
Language:English
Subjects:
Animals
Babesia bovis
Babesia bovis - growth & development
Babesia bovis - ultrastructure
Biological and medical sciences
Blood Preservation - veterinary
Cattle
cell culture
Cells, Cultured
Cryopreservation
Cryopreservation - veterinary
cultivo de celulas
culture de cellule
Culture Media
Deer
Deer - blood
Erythrocytes
Erythrocytes - parasitology
Erythrocytes - ultrastructure
Fundamental and applied biological sciences. Psychology
General aspects and techniques
In vitro Cultivation
Invertebrates
Male
Microscopy, Electron
Odocoileus virginianus
Parasitemia
Parasites
Parasitism
Parasitology
Protozoa
Subcultures
Ticks
Ungulates
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Description
Summary:Babesia bovis cultured in bovine erythrocytes was passaged into white-tailed deer (Odocoileus virginianus) erythrocytes and medium containing either white-tailed deer serum or bovine serum. Deer erythrocytes supported the growth of the parasite only in the presence of bovine serum. Cryopreserved cultures were recovered successfully in white-tailed deer erythrocytes. By light and electron microscopy, B. bovis structure appeared similar in host cells of either species.
ISSN:0022-3395
1937-2345
DOI:10.2307/3283513