Cloning and characterization of heat‐inducible promoters of Bacillus subtilis

Heat‐inducible DNA fragments of Bacillus subtilis were cloned with two different promoter probe vectors. The increased synthesis of the reporter enzymes seemed to be due to a transient increase in the transcription of the encoding genes. The structures of the heat‐sensitive promoters resembles the c...

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Bibliographic Details
Published in:FEMS microbiology letters 1993-02, Vol.106 (3), p.287-293
Main Authors: Völker, Uwe, Riethdorf, Sabine, Winkler, Anett, Weigend, Bettina, Fortnagel, Peter, Hecker, Michael
Format: Article
Language:English
Subjects:
Bacillus subtilis
Bacillus subtilis - genetics
Bacteriology
Base Sequence
Biological and medical sciences
Catechol 2,3-Dioxygenase
Chromosome Mapping
Cloning, Molecular
Dioxygenases
DNA Probes
DNA, Bacterial - genetics
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Genetic Vectors
Genetics
Glycoside Hydrolases - genetics
Heat shock
Heat-Shock Proteins - genetics
Hot Temperature
Microbiology
Molecular Sequence Data
Oxygenases - genetics
Promoter Regions, Genetic
Promoter structure
Sigma A
Sigma Factor - genetics
Transcription, Genetic
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Summary:Heat‐inducible DNA fragments of Bacillus subtilis were cloned with two different promoter probe vectors. The increased synthesis of the reporter enzymes seemed to be due to a transient increase in the transcription of the encoding genes. The structures of the heat‐sensitive promoters resembles the consensus sequence of promoters recognized by the vegetative form of RNA polymerase of B. subtilis. Our results support data in literature that the heat shock response of B. subtilis is regulated by a different mechanism than in Escherichia coli, where alternative sigma factors direct the transcription of heat shock genes.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1993.tb05978.x