Interleukin-1-dependent mitogenic responses induced by protoscoleces of Echinococcus multilocularis in murine lymphocytes

• Published in: Journal of leukocyte biology 1993-03, Vol.53 (3), p.233-239
• Main Authors: KIZAKI, T, ISHIGE, M, BINGYAN, W, KUMAGAI, M, DAY, N. K, GOOD, R. A, ONOE, K
• Format: Article
• Language: English
• Subjects: Analysis of the immune response. Humoral and cellular immunity; Animals; B-Lymphocytes - parasitology; Biological and medical sciences; Cell Adhesion; Echinococcosis - parasitology; Echinococcus - drug effects; Echinococcus - immunology; Echinococcus multilocularis; Female; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Immune Sera - pharmacology; Immunobiology; Interleukin-1 - immunology; Interleukin-1 - pharmacology; Lymphocyte Activation - immunology; Lymphocyte Subsets - parasitology; Lymphocytes - parasitology; Lymphokines, interleukins ( function, expression); Macrophages - metabolism; Mice; Mice, Inbred BALB C; Mice, Inbred C3H; Mitogens - immunology; Monocytes - metabolism; Regulatory factors and their cellular receptors; Spleen - cytology; Spleen - metabolism; Spleen - parasitology; T-Lymphocytes - parasitology
• ISSN: 0741-5400; 1938-3673
• DOI: 10.1002/jlb.53.3.233

Mitogenic effects of protoscoleces (PSCs) of Echinococcus multilocularis on murine lymphocytes were studied. Spleen cells from normal BALB/c mice showed significant proliferative responses when cocultured with PSCs. Proliferative responses were observed in both the T and B cell populations. The PSCs also stimulated cells of the macrophage/monocyte lineage to secrete interleukin-1 (IL-1). Depletion of plastic- and Sephadex G-10-adherent cells from the spleen cell population significantly reduced the proliferative responses to PSCs and the low responsiveness was restored by addition of plastic-adherent cells to these cultures. Furthermore, addition of anti-IL-1 serum to the spleen cell cultures stimulated with PSCs completely suppressed the proliferative responses. These findings demonstrate that the mitogenic effect of PSC on lymphocytes depends on IL-1 secreted by cells of macrophage/monocyte lineage.