Nucleotide sequence of cDNA encoding the fire ant venom protein Sol i II

For the first time the cDNA encoding a fire ant venom protein has been sequenced. Oligonucleotides were designed according to the amino acid sequence. The cDNA sequence was obtained by hybridizing these primers to mRNA and enhancement by the PCR technique. Comparison to the amino acid sequence of th...

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Bibliographic Details
Published in:FEBS letters 1993-03, Vol.319 (1), p.138-140
Main Authors: Schmidt, Margit, Walker, Robert B., Hoffman, Donald R., McConnell, Thomas J.
Format: Article
Language:English
Subjects:
Allergy
Amino Acid Sequence
Analytical, structural and metabolic biochemistry
Ant Venoms - chemistry
Ant Venoms - genetics
Base Sequence
Biological and medical sciences
cDNA cloning
DNA - chemistry
DNA - genetics
Fundamental and applied biological sciences. Psychology
Insect Proteins
Insect venom
Miscellaneous
Molecular Sequence Data
Nucleic Acid Hybridization
PCR
Polymerase Chain Reaction
Proteins
Transformation, Bacterial
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Description
Summary:For the first time the cDNA encoding a fire ant venom protein has been sequenced. Oligonucleotides were designed according to the amino acid sequence. The cDNA sequence was obtained by hybridizing these primers to mRNA and enhancement by the PCR technique. Comparison to the amino acid sequence of the venom protein shows a leader sequence 19 amino acids long.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(93)80053-W