In vitro binding to the leucine tRNA gene identifies a novel yeast homeobox gene

In a search for gene products of Saccharomyces cerevisiae interacting with the internal promoter of yeast tRNA genes two genes encoding a homeodomain protein of the Drosophila Antennapedia type were isolated. One of them codes for Pho2, and the second codes for a previously unknown protein (Yox1). T...

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Bibliographic Details
Published in:Chromosoma 1993-02, Vol.102 (3), p.174-179
Main Author: Kaufmann, E
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acid sequences
Base Sequence
binding sites
Biological and medical sciences
Cell Cycle Proteins
DNA, Fungal - genetics
DNA, Fungal - metabolism
DNA-binding proteins
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Fundamental and applied biological sciences. Psychology
Fungal Proteins - genetics
genbank/x62392
genes
Genes, Fungal
Genes, Homeobox
Genes. Genome
Homeodomain Proteins
leucine
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
nucleotide sequences
promoter regions
Repressor Proteins
RNA, Transfer, Leu - genetics
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins
Sequence Homology, Amino Acid
structural genes
Trans-Activators - genetics
transfer RNA
yox1 gene
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Summary:In a search for gene products of Saccharomyces cerevisiae interacting with the internal promoter of yeast tRNA genes two genes encoding a homeodomain protein of the Drosophila Antennapedia type were isolated. One of them codes for Pho2, and the second codes for a previously unknown protein (Yox1). The corresponding gene, termed YOX1, maps to chromosome 16. The amino acid sequence of Yox1 shows a remarkable similarity within the homeobox domain to many proteins from a wide variety of sources. Fusion proteins that contain sequences encoded by these genes demonstrate that the genes encode DNA-binding proteins that are capable of binding to the DNA of the leucine tRNA gene in vitro. However, deletion of YOX1 gene activity does not give rise to a scorable mutant phenotype. This result leaves open whether Yox1 binding to the leucine tRNA gene is necessary for the in vivo regulation of the gene and it suggests that the YOX1 gene codes for a nonessential product.
ISSN:0009-5915
1432-0886
DOI:10.1007/BF00387732