Amplified bcl-2/JH rearrangements in reactive lymphadenopathy

Using the polymerase chain reaction (PCR) to examine the occurrence of bcl-2/JH joining produced by t(14;18) chromosomal translocation, amplified DNA was detected in 2 of 18 lymph nodes showing reactive lymphadenopathy. The PCR was repeated in these two lymph nodes using the same DNA samples, but no...

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Bibliographic Details
Published in:Virchows Archiv. B, Cell pathology Cell pathology, 1993, Vol.63 (3), p.197-198
Main Authors: OHSHIMA, K, KIKUCHI, M, KOBARI, S, MASUDA, Y, EGUCHI, F, KIMURA, N
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Chromosomes, Human, Pair 14
Chromosomes, Human, Pair 18
DNA - analysis
Gene Rearrangement
Hematologic and hematopoietic diseases
Humans
Immunoglobulin Heavy Chains - genetics
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
Lymphoproliferative Disorders - genetics
Medical sciences
Molecular Sequence Data
Polymerase Chain Reaction
Proto-Oncogene Proteins - genetics
Proto-Oncogene Proteins c-bcl-2
Translocation, Genetic
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Summary:Using the polymerase chain reaction (PCR) to examine the occurrence of bcl-2/JH joining produced by t(14;18) chromosomal translocation, amplified DNA was detected in 2 of 18 lymph nodes showing reactive lymphadenopathy. The PCR was repeated in these two lymph nodes using the same DNA samples, but no amplification was detected at the second attempt. Thus the amplified DNA was considered to be derived from one copy of joined bcl-2/JH in one cell, or from a few copies in a few clonal cells with the same joined bcl-2/JH. These results suggest that false joining of bcl-2/JH at the t(14;18) junction may occur in reactive lymph nodes.
ISSN:0340-6075
DOI:10.1007/BF02899261