Frequency and Phenotype of T Helper 17 Cells in Peripheral Blood and Synovial Fluid of Patients with Reactive Arthritis

To analyze the frequency and phenotype of peripheral blood mononuclear cells (PBMC) and synovial fluid mononuclear cell (SFMC) T helper (Th)17 cells in reactive arthritis (ReA). T cell surface phenotype and cytokine production were measured following stimulation, using 8-color flow cytometry. The pe...

Full description

Saved in:
Bibliographic Details
Published in:Journal of rheumatology 2010-10, Vol.37 (10), p.2096-2099
Main Authors: HUI SHEN, GOODALL, Jane C, GASTON, J. S. Hill
Format: Article
Language:English
Subjects:
Arthritis, Reactive - blood
Arthritis, Reactive - immunology
Biological and medical sciences
CD4-Positive T-Lymphocytes - immunology
Diseases of the osteoarticular system
Diseases of the spine
Humans
Inflammatory joint diseases
Interleukin-17 - immunology
Medical sciences
Phenotype
Synovial Fluid - cytology
Synovial Fluid - immunology
T-Lymphocytes, Helper-Inducer - immunology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To analyze the frequency and phenotype of peripheral blood mononuclear cells (PBMC) and synovial fluid mononuclear cell (SFMC) T helper (Th)17 cells in reactive arthritis (ReA). T cell surface phenotype and cytokine production were measured following stimulation, using 8-color flow cytometry. The percentages of interleukin 17 (IL-17)-positive CD4+ T cells were increased in SFMC of patients with ReA compared with PBMC. All IL-17+ cells were CD4+CD45RO+; in SFMC most expressed CCR6, but only 50% expressed CCR4. IL-17+ cells sometimes coexpressed IL-22 and/or interferon-γ, but not IL-10. These data support the hypothesis that Th17 cells are involved in ReA pathogenesis.
ISSN:0315-162X
1499-2752
DOI:10.3899/jrheum.100146