Homology Modeling and Docking Studies of Human Bcl-2L10 Protein

Cancer, an unrestrained proliferation of cells, is one of the lead cause of death. Nearly 12.5 million people are diagnosed with cancer worldwide, 7.5 million people die of which 2.5 million cases are from India. Major cause for cancer is restriction of programmed cell death (apoptosis). Multiple si...

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Bibliographic Details
Published in:Journal of biomolecular structure & dynamics 2010-12, Vol.28 (3), p.379-391
Main Authors: Bhargavi, K., Chaitanya, P. Kalyan, Ramasree, D., Vasavi, M., Murthy, D. K., Uma, V.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Apoptosis
Bcl-2
bcl-2-Associated X Protein - chemistry
bcl-2-Associated X Protein - genetics
bcl-2-Associated X Protein - metabolism
Bcl-2L10
BH3 domain
Cancer therapy
Catalytic Domain
Comparative Modeling
Dimerization
Humans
Models, Molecular
Molecular Docking
Molecular Dynamics Simulation
Molecular Sequence Data
Protein Binding
Protein Structure, Tertiary
Proto-Oncogene Proteins c-bcl-2 - chemistry
Proto-Oncogene Proteins c-bcl-2 - genetics
Proto-Oncogene Proteins c-bcl-2 - metabolism
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Summary:Cancer, an unrestrained proliferation of cells, is one of the lead cause of death. Nearly 12.5 million people are diagnosed with cancer worldwide, 7.5 million people die of which 2.5 million cases are from India. Major cause for cancer is restriction of programmed cell death (apoptosis). Multiple signaling pathways regulate apoptosis. Bcl-2 (B-Cell Lymphomas-2) family proteins play a vital role as central regulators of apoptosis. Bcl-2L10, a novel anti- apoptotic protein, blocks apoptosis by mitochondrial dependent mechanism. The present study evaluates the 3D structure of Bcl-2L10 protein using homology modeling and aims to understand plausible functional and binding interactions between Bcl-2L10 with BH3 domain of BAX using protein-protein docking. The docking studies show binding of BH3 domain at Lys 110, Trp-111, Pro-115, Glu-119 and Asp-127 in the groove of BH 1, 2 and 3 domains of Bcl-2L10. Heterodimerization of anti-apoptotic Bcl-2 and BH3 domain of pro- apoptotic Bcl-2 proteins instigates apoptosis. Profound understanding of Bcl-2 pathway may prove useful in identification of future therapeutic targets for cancer.
ISSN:0739-1102
1538-0254
DOI:10.1080/07391102.2010.10507367