Inducible Operation of the Erythropoietin 3' Enhancer in Multiple Cell Lines: Evidence for a Widespread Oxygen-Sensing Mechanism

Adaptive responses to hypoxia occur in many biological systems. A well-characterized example is the hypoxic induction of the synthesis of erythropoietin, a hormone which regulates erythropoiesis and hence blood oxygen content. The restricted expression of the erythropoietin gene in subsets of cells...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1993-03, Vol.90 (6), p.2423-2427
Main Authors: Maxwell, P. H., Pugh, C. W., Ratcliffe, P. J.
Format: Article
Language:English
Subjects:
Anatomy & physiology
Animals
Biological and medical sciences
Cell Hypoxia
Cell Line
Cell lines
Cellular biology
CHO cells
Cobalt - pharmacology
COS cells
Enhancer Elements, Genetic
Epics
Erythropoietin - genetics
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Regulation - drug effects
Genes
Humans
Hypoxia
LLC PK1 cells
Mammals
Molecular and cellular biology
Molecular genetics
Organ Specificity
Oxygen - physiology
Plasmids
Restriction Mapping
RNA
Shock heating
Transfection
Tumor Cells, Cultured
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Adaptive responses to hypoxia occur in many biological systems. A well-characterized example is the hypoxic induction of the synthesis of erythropoietin, a hormone which regulates erythropoiesis and hence blood oxygen content. The restricted expression of the erythropoietin gene in subsets of cells within kidney and liver has suggested that this specific oxygen-sensing mechanism is restricted to specialized cells in those organs. Using transient transfection of reporter genes coupled to a transcriptional enhancer lying 3' to the erythropoietin gene, we show that an oxygen-sensing system similar, or identical, to that controlling erythropoietin expression is widespread in mammalian cells. The extensive distribution of this sensing mechanism contrasts with the restricted expression of erythropoietin, suggesting that it mediates other adaptive responses to hypoxia.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.90.6.2423