Synthesis and assembly of infectious bovine papillomavirus particles in vitro

1 Papillomavirus Research Unit, Lions Human Immunology Laboratories, Princess Alexandra Hospital, Ipswich Road, Brisbane, Queensland 4102 and 2 Analytical Electron Microscopy Facility, Queensland University of Technology, George Street, Brisbane, Queensland 4001, Australia Bovine papillomavirus type...

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Bibliographic Details
Published in:Journal of general virology 1993-04, Vol.74 (4), p.763-768
Main Authors: Zhou, Jian, Stenzel, Deborah J, Sun, Xiao-Yi, Frazer, Ian H
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Bovine papillomavirus 1 - growth & development
Bovine papillomavirus 1 - ultrastructure
Capsid - genetics
Capsid - metabolism
Cattle
Cloning, Molecular
Fundamental and applied biological sciences. Psychology
In Vitro Techniques
Microbiology
Molecular Sequence Data
Oligodeoxyribonucleotides - chemistry
Recombinant Proteins - metabolism
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
Vaccinia virus
Virology
Virus Replication
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Summary:1 Papillomavirus Research Unit, Lions Human Immunology Laboratories, Princess Alexandra Hospital, Ipswich Road, Brisbane, Queensland 4102 and 2 Analytical Electron Microscopy Facility, Queensland University of Technology, George Street, Brisbane, Queensland 4001, Australia Bovine papillomavirus type 1 (BPV-1) virions were produced in vitro using vaccinia virus (VV) recombinants expressing the BPV-1 L1 and L2 capsid proteins. Particles morphologically resembling papillomaviruses were observed in the nucleus of cells infected with a VV recombinant for the BPV-1 L1 protein, and greater numbers of similar particles were seen in the nuclei of cells infected with a VV double recombinant for L1 and L2. Virus-like particles (VLPs) assembled in cells infected with the VV double recombinant for BPV-1 L1 and L2, and not those assembled in cells infected with the VV recombinant for BPV-1 L1 alone, were able to package BPV-1 DNA. Transcription of the BPV-1 E1 viral open reading frame was observed after a mouse fibroblast cell line was exposed to VLPs produced using a BPV-1 L1/L2 VV recombinant in a cell line containing episomal BPV-1 DNA. E1 transcription was not observed when the VLPs were pre-incubated with antibodies to the capsid protein of BPV-1. This system should allow an in vitro approach to the definition of the BPV-1 cellular receptor. Received 21 September 1992; accepted 1 December 1992.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-74-4-763