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Synthesis and assembly of infectious bovine papillomavirus particles in vitro
1 Papillomavirus Research Unit, Lions Human Immunology Laboratories, Princess Alexandra Hospital, Ipswich Road, Brisbane, Queensland 4102 and 2 Analytical Electron Microscopy Facility, Queensland University of Technology, George Street, Brisbane, Queensland 4001, Australia Bovine papillomavirus type...
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| Published in: | Journal of general virology 1993-04, Vol.74 (4), p.763-768 |
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| container_end_page | 768 |
| container_issue | 4 |
| container_start_page | 763 |
| container_title | Journal of general virology |
| container_volume | 74 |
| creator | Zhou, Jian Stenzel, Deborah J Sun, Xiao-Yi Frazer, Ian H |
| description | 1 Papillomavirus Research Unit, Lions Human Immunology Laboratories, Princess Alexandra Hospital, Ipswich Road, Brisbane, Queensland 4102
and 2 Analytical Electron Microscopy Facility, Queensland University of Technology, George Street, Brisbane, Queensland 4001, Australia
Bovine papillomavirus type 1 (BPV-1) virions were produced in vitro using vaccinia virus (VV) recombinants expressing the BPV-1 L1 and L2 capsid proteins. Particles morphologically resembling papillomaviruses were observed in the nucleus of cells infected with a VV recombinant for the BPV-1 L1 protein, and greater numbers of similar particles were seen in the nuclei of cells infected with a VV double recombinant for L1 and L2. Virus-like particles (VLPs) assembled in cells infected with the VV double recombinant for BPV-1 L1 and L2, and not those assembled in cells infected with the VV recombinant for BPV-1 L1 alone, were able to package BPV-1 DNA. Transcription of the BPV-1 E1 viral open reading frame was observed after a mouse fibroblast cell line was exposed to VLPs produced using a BPV-1 L1/L2 VV recombinant in a cell line containing episomal BPV-1 DNA. E1 transcription was not observed when the VLPs were pre-incubated with antibodies to the capsid protein of BPV-1. This system should allow an in vitro approach to the definition of the BPV-1 cellular receptor.
Received 21 September 1992;
accepted 1 December 1992. |
| doi_str_mv | 10.1099/0022-1317-74-4-763 |
| format | article |
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and 2 Analytical Electron Microscopy Facility, Queensland University of Technology, George Street, Brisbane, Queensland 4001, Australia
Bovine papillomavirus type 1 (BPV-1) virions were produced in vitro using vaccinia virus (VV) recombinants expressing the BPV-1 L1 and L2 capsid proteins. Particles morphologically resembling papillomaviruses were observed in the nucleus of cells infected with a VV recombinant for the BPV-1 L1 protein, and greater numbers of similar particles were seen in the nuclei of cells infected with a VV double recombinant for L1 and L2. Virus-like particles (VLPs) assembled in cells infected with the VV double recombinant for BPV-1 L1 and L2, and not those assembled in cells infected with the VV recombinant for BPV-1 L1 alone, were able to package BPV-1 DNA. Transcription of the BPV-1 E1 viral open reading frame was observed after a mouse fibroblast cell line was exposed to VLPs produced using a BPV-1 L1/L2 VV recombinant in a cell line containing episomal BPV-1 DNA. E1 transcription was not observed when the VLPs were pre-incubated with antibodies to the capsid protein of BPV-1. This system should allow an in vitro approach to the definition of the BPV-1 cellular receptor.
Received 21 September 1992;
accepted 1 December 1992.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-74-4-763</identifier><identifier>PMID: 8385700</identifier><identifier>CODEN: JGVIAY</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Animals ; Base Sequence ; Biological and medical sciences ; Bovine papillomavirus 1 - growth & development ; Bovine papillomavirus 1 - ultrastructure ; Capsid - genetics ; Capsid - metabolism ; Cattle ; Cloning, Molecular ; Fundamental and applied biological sciences. Psychology ; In Vitro Techniques ; Microbiology ; Molecular Sequence Data ; Oligodeoxyribonucleotides - chemistry ; Recombinant Proteins - metabolism ; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains ; Vaccinia virus ; Virology ; Virus Replication</subject><ispartof>Journal of general virology, 1993-04, Vol.74 (4), p.763-768</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3183-136c23d18b51662b5d7e7de373fe75e373c55b03635de4ccf7fc3ff3c09703ab3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4781459$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8385700$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhou, Jian</creatorcontrib><creatorcontrib>Stenzel, Deborah J</creatorcontrib><creatorcontrib>Sun, Xiao-Yi</creatorcontrib><creatorcontrib>Frazer, Ian H</creatorcontrib><title>Synthesis and assembly of infectious bovine papillomavirus particles in vitro</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>1 Papillomavirus Research Unit, Lions Human Immunology Laboratories, Princess Alexandra Hospital, Ipswich Road, Brisbane, Queensland 4102
and 2 Analytical Electron Microscopy Facility, Queensland University of Technology, George Street, Brisbane, Queensland 4001, Australia
Bovine papillomavirus type 1 (BPV-1) virions were produced in vitro using vaccinia virus (VV) recombinants expressing the BPV-1 L1 and L2 capsid proteins. Particles morphologically resembling papillomaviruses were observed in the nucleus of cells infected with a VV recombinant for the BPV-1 L1 protein, and greater numbers of similar particles were seen in the nuclei of cells infected with a VV double recombinant for L1 and L2. Virus-like particles (VLPs) assembled in cells infected with the VV double recombinant for BPV-1 L1 and L2, and not those assembled in cells infected with the VV recombinant for BPV-1 L1 alone, were able to package BPV-1 DNA. Transcription of the BPV-1 E1 viral open reading frame was observed after a mouse fibroblast cell line was exposed to VLPs produced using a BPV-1 L1/L2 VV recombinant in a cell line containing episomal BPV-1 DNA. E1 transcription was not observed when the VLPs were pre-incubated with antibodies to the capsid protein of BPV-1. This system should allow an in vitro approach to the definition of the BPV-1 cellular receptor.
Received 21 September 1992;
accepted 1 December 1992.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Bovine papillomavirus 1 - growth & development</subject><subject>Bovine papillomavirus 1 - ultrastructure</subject><subject>Capsid - genetics</subject><subject>Capsid - metabolism</subject><subject>Cattle</subject><subject>Cloning, Molecular</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In Vitro Techniques</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Oligodeoxyribonucleotides - chemistry</subject><subject>Recombinant Proteins - metabolism</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>Vaccinia virus</subject><subject>Virology</subject><subject>Virus Replication</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><recordid>eNo9kF1LwzAUhoMoc07_gCD0QvSqmjRJ017K8AsmXqjXIU1PXKRtatJN9u9NWdnVgbzPOXl5ELok-I7gsrzHOMtSQolIBUtZKnJ6hOaE5TzNYnyM5gfgFJ2F8IMxYYyLGZoVtOAC4zl6-9h1wxqCDYnq6kSFAG3V7BJnEtsZ0IN1m5BUbms7SHrV26ZxrdpaH1975QerGwgRTbZ28O4cnRjVBLiY5gJ9PT1-Ll_S1fvz6_JhlWpKChob5TqjNSkqTvI8q3gtQNRABTUg-Dg15xWmOeU1MK2NMJoaQzUuBaaqogt0s7_be_e7gTDI1gYNTaM6iH2l4LmgJaMRzPag9i4ED0b23rbK7yTBcnQoR0VyVCQFk0xGh3Hparq-qVqoDyuTtJhfT7kKWjXGq07bcMCYKAjjZcRu99jafq__rAf5DV1rY5PKOhkVHj78Bxo9h8g</recordid><startdate>199304</startdate><enddate>199304</enddate><creator>Zhou, Jian</creator><creator>Stenzel, Deborah J</creator><creator>Sun, Xiao-Yi</creator><creator>Frazer, Ian H</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199304</creationdate><title>Synthesis and assembly of infectious bovine papillomavirus particles in vitro</title><author>Zhou, Jian ; Stenzel, Deborah J ; Sun, Xiao-Yi ; Frazer, Ian H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3183-136c23d18b51662b5d7e7de373fe75e373c55b03635de4ccf7fc3ff3c09703ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Bovine papillomavirus 1 - growth & development</topic><topic>Bovine papillomavirus 1 - ultrastructure</topic><topic>Capsid - genetics</topic><topic>Capsid - metabolism</topic><topic>Cattle</topic><topic>Cloning, Molecular</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In Vitro Techniques</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Oligodeoxyribonucleotides - chemistry</topic><topic>Recombinant Proteins - metabolism</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>Vaccinia virus</topic><topic>Virology</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhou, Jian</creatorcontrib><creatorcontrib>Stenzel, Deborah J</creatorcontrib><creatorcontrib>Sun, Xiao-Yi</creatorcontrib><creatorcontrib>Frazer, Ian H</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhou, Jian</au><au>Stenzel, Deborah J</au><au>Sun, Xiao-Yi</au><au>Frazer, Ian H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthesis and assembly of infectious bovine papillomavirus particles in vitro</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1993-04</date><risdate>1993</risdate><volume>74</volume><issue>4</issue><spage>763</spage><epage>768</epage><pages>763-768</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><coden>JGVIAY</coden><abstract>1 Papillomavirus Research Unit, Lions Human Immunology Laboratories, Princess Alexandra Hospital, Ipswich Road, Brisbane, Queensland 4102
and 2 Analytical Electron Microscopy Facility, Queensland University of Technology, George Street, Brisbane, Queensland 4001, Australia
Bovine papillomavirus type 1 (BPV-1) virions were produced in vitro using vaccinia virus (VV) recombinants expressing the BPV-1 L1 and L2 capsid proteins. Particles morphologically resembling papillomaviruses were observed in the nucleus of cells infected with a VV recombinant for the BPV-1 L1 protein, and greater numbers of similar particles were seen in the nuclei of cells infected with a VV double recombinant for L1 and L2. Virus-like particles (VLPs) assembled in cells infected with the VV double recombinant for BPV-1 L1 and L2, and not those assembled in cells infected with the VV recombinant for BPV-1 L1 alone, were able to package BPV-1 DNA. Transcription of the BPV-1 E1 viral open reading frame was observed after a mouse fibroblast cell line was exposed to VLPs produced using a BPV-1 L1/L2 VV recombinant in a cell line containing episomal BPV-1 DNA. E1 transcription was not observed when the VLPs were pre-incubated with antibodies to the capsid protein of BPV-1. This system should allow an in vitro approach to the definition of the BPV-1 cellular receptor.
Received 21 September 1992;
accepted 1 December 1992.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>8385700</pmid><doi>10.1099/0022-1317-74-4-763</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of general virology, 1993-04, Vol.74 (4), p.763-768 |
| issn | 0022-1317 1465-2099 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75673943 |
| source | Freely Accessible Journals |
| subjects | Animals Base Sequence Biological and medical sciences Bovine papillomavirus 1 - growth & development Bovine papillomavirus 1 - ultrastructure Capsid - genetics Capsid - metabolism Cattle Cloning, Molecular Fundamental and applied biological sciences. Psychology In Vitro Techniques Microbiology Molecular Sequence Data Oligodeoxyribonucleotides - chemistry Recombinant Proteins - metabolism Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Vaccinia virus Virology Virus Replication |
| title | Synthesis and assembly of infectious bovine papillomavirus particles in vitro |
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