delta 9-Tetrahydrocannabinol by GLC-MS validated radioimmunoassays of hemolyzed blood or serum

A direct radioimmunoassay (RIA) of delta 9-tetrahydrocannabinol (delta 9-THC) in unextracted hemolyzed blood or serum is described. The dose-response curve in the range of 5-50 ng/mL (serum or blood) was linear on log-logit transformation and iterative weighted regression. Validation studies include...

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Bibliographic Details
Published in:Journal of analytical toxicology 1981-03, Vol.5 (2), p.81-84
Main Authors: Yeager, E P, Goebelsmann, U, Soares, J R, Grant, J D, Gross, S J
Format: Article
Language:English
Subjects:
Animals
Antibody Specificity
Cannabinoids - blood
Dronabinol - blood
Dronabinol - immunology
Gas Chromatography-Mass Spectrometry - methods
Goats - immunology
Humans
Radioimmunoassay - methods
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Summary:A direct radioimmunoassay (RIA) of delta 9-tetrahydrocannabinol (delta 9-THC) in unextracted hemolyzed blood or serum is described. The dose-response curve in the range of 5-50 ng/mL (serum or blood) was linear on log-logit transformation and iterative weighted regression. Validation studies included testing for precision, accuracy and antibody specificity as well as confirmation of RIA results (marijuana smoker samples) by gas liquid chromatography-mass spectroscopy (GLC-MS). This routine method will greatly augment cannabinoid investigative programs.
ISSN:0146-4760
DOI:10.1093/jat/5.2.81