Identification of three wx proteins in wheat (Triticum aestivum L.)

Nullisomic analysis of waxy (Wx) protein of hexaploid wheat (Triticum aestivum L.) cv. "Chinese Spring" using two-dimensional polyacrylamide gel electrophoresis revealed that three Wx loci, Wx-A1, Wx-B1, and Wx-D1, located on chromosome arms 7AS, 4AL, and 7DS, produce three distinct Wx sub...

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Bibliographic Details
Published in:Biochemical genetics 1993-02, Vol.31 (1-2), p.75-86
Main Authors: NAKAMURA, T, YAMAMORI, M, HIRANO, H, HIDAKA, S
Format: Article
Language:English
Subjects:
Biological and medical sciences
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
Gene Expression
Genes, Plant
Molecular and cellular biology
Molecular genetics
Molecular Weight
Phenotype
Plant Proteins - analysis
Plant Proteins - chemistry
Plant Proteins - genetics
Starch Synthase
Triticum - chemistry
Triticum - genetics
Triticum - metabolism
Triticum aestivum
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Summary:Nullisomic analysis of waxy (Wx) protein of hexaploid wheat (Triticum aestivum L.) cv. "Chinese Spring" using two-dimensional polyacrylamide gel electrophoresis revealed that three Wx loci, Wx-A1, Wx-B1, and Wx-D1, located on chromosome arms 7AS, 4AL, and 7DS, produce three distinct Wx subunit groups, subunit group-A (SGA), SGB, and SGD, respectively. SGA has a higher molecular weight and a more basic isoelectric point (pI) than the other two. SGB and SGD have the same molecular weight but a slightly different pI range. Owing to the detection of these three subunit groups, we were able to identify the expression of three waxy genes in wheat endosperm and to find two types of mutants among Japanese wheat cultivars, one lacking SGA and the others SGB. These results suggest the possibility of breeding a waxy wheat.
ISSN:0006-2928
1573-4927
DOI:10.1007/bf02399821