Identification of three wx proteins in wheat (Triticum aestivum L.)

• Published in: Biochemical genetics 1993-02, Vol.31 (1-2), p.75-86
• Main Authors: NAKAMURA, T, YAMAMORI, M, HIRANO, H, HIDAKA, S
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Electrophoresis, Polyacrylamide Gel; Fundamental and applied biological sciences. Psychology; Gene Expression; Genes, Plant; Molecular and cellular biology; Molecular genetics; Molecular Weight; Phenotype; Plant Proteins - analysis; Plant Proteins - chemistry; Plant Proteins - genetics; Starch Synthase; Triticum - chemistry; Triticum - genetics; Triticum - metabolism; Triticum aestivum
• ISSN: 0006-2928; 1573-4927
• DOI: 10.1007/bf02399821

Nullisomic analysis of waxy (Wx) protein of hexaploid wheat (Triticum aestivum L.) cv. "Chinese Spring" using two-dimensional polyacrylamide gel electrophoresis revealed that three Wx loci, Wx-A1, Wx-B1, and Wx-D1, located on chromosome arms 7AS, 4AL, and 7DS, produce three distinct Wx subunit groups, subunit group-A (SGA), SGB, and SGD, respectively. SGA has a higher molecular weight and a more basic isoelectric point (pI) than the other two. SGB and SGD have the same molecular weight but a slightly different pI range. Owing to the detection of these three subunit groups, we were able to identify the expression of three waxy genes in wheat endosperm and to find two types of mutants among Japanese wheat cultivars, one lacking SGA and the others SGB. These results suggest the possibility of breeding a waxy wheat.