Receptor Activator of Nuclear Factor‐Kappa B Ligand/Osteoprotegerin Ratio in Sites of Chronic Periodontitis of Subjects With Poorly and Well‐Controlled Type 2 Diabetes

• Published in: Journal of periodontology (1970) 2010-10, Vol.81 (10), p.1455-1465
• Main Authors: Santos, Vanessa Renata, Lima, Jadson Almeida, Gonçalves, Tiago Eduardo Dias, Bastos, Marta Ferreira, Figueiredo, Luciene Cristina, Shibli, Jamil Awad, Duarte, Poliana Mendes
• Format: Article
• Language: English
• Subjects: Adult; Aged; Analysis of Variance; Biological and medical sciences; Chi-Square Distribution; Chronic periodontitis; Chronic Periodontitis - complications; Chronic Periodontitis - metabolism; Chronic Periodontitis - therapy; Dental Plaque - therapy; Dental Scaling; Dentistry; diabetes mellitus; Diabetes Mellitus, Type 2 - blood; Diabetes Mellitus, Type 2 - complications; Diabetes Mellitus, Type 2 - metabolism; Diabetes. Impaired glucose tolerance; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Etiopathogenesis. Screening. Investigations. Target tissue resistance; Facial bones, jaws, teeth, parodontium: diseases, semeiology; Female; Gingival Crevicular Fluid - chemistry; Glycated Hemoglobin A - analysis; Humans; Male; Medical sciences; Middle Aged; Non tumoral diseases; osteoprotegerin; Osteoprotegerin - analysis; Osteoprotegerin - metabolism; Otorhinolaryngology. Stomatology; RANK ligand; RANK Ligand - analysis; RANK Ligand - metabolism; root planing; Statistics, Nonparametric
• ISSN: 0022-3492; 1943-3670
• DOI: 10.1902/jop.2010.100125

Background: The aim of this study is to evaluate the levels of osteoclastogenesis‐related factors (soluble receptor activator of nuclear factor‐kappa B ligand [sRANKL] and osteoprotegerin [OPG]) in gingival crevicular fluid (GCF) from subjects with poorly and well‐controlled type 2 diabetes and chronic periodontitis before and after periodontal therapy. Methods: Eighteen subjects with well‐controlled diabetes (glycated hemoglobin [HbA1c] levels ≤8%) and 20 subjects with poorly controlled diabetes (HbA1c levels >8%) were enrolled in this study. All subjects were submitted to non‐surgical periodontal therapy. GCF sampling and clinical periodontal parameters were assessed at baseline and 3 and 6 months post‐therapy. Total amounts and concentrations of sRANKL and OPG in GCF were analyzed by enzyme‐linked immunosorbent assay (ELISA). Results: Total amounts and concentrations of sRANKL and RANKL/OPG ratios were higher in poorly controlled subjects than in well‐controlled subjects at baseline and 3 and 6 months post‐therapy (P