Construction of Two BAC Libraries from Half-Smooth Tongue Sole Cynoglossus semilaevis and Identification of Clones Containing Candidate Sex-Determination Genes

Half-smooth tongue sole ( Cynoglossus semilaevis ) is an increasingly important aquaculture species in China. It is also a tractable model to study sex chromosome evolution and to further elucidate the mechanism of sex determination in teleosts. Two bacterial artificial chromosome (BAC) libraries fo...

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Bibliographic Details
Published in:Marine biotechnology (New York, N.Y.) N.Y.), 2010-10, Vol.12 (5), p.558-568
Main Authors: Shao, Chang-Wei, Chen, Song-Lin, Scheuring, Chantel F., Xu, Jian-Yong, Sha, Zhen-Xia, Dong, Xiao-Li, Zhang, Hong-Bin
Format: Article
Language:English
Subjects:
Animals
Aquaculture
Arrays
Artificial chromosomes
Bacteria
Bacterial artificial chromosomes
Biomedical and Life Sciences
Biotechnology
Chromosomes, Artificial, Bacterial - genetics
Clones
Cloning
Cloning, Molecular
Construction
DNA probes
Engineering
Female
Fisheries - methods
Flatfishes - classification
Flatfishes - genetics
Freshwater & Marine Ecology
Gene Expression Regulation - genetics
Gene Library
Genes
Genomes
Haploidy
Inserts
International
Laboratory equipment
Life Sciences
Male
Marine biology
Microbiology
Original Article
Plates
Probability theory
Sequencing
Sex
Sex determination
Sex Determination Processes - genetics
Studies
Zoology
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Summary:Half-smooth tongue sole ( Cynoglossus semilaevis ) is an increasingly important aquaculture species in China. It is also a tractable model to study sex chromosome evolution and to further elucidate the mechanism of sex determination in teleosts. Two bacterial artificial chromosome (BAC) libraries for C . semilaevis , with large, high-quality inserts and deep coverage, were constructed in the Bam HI and Hind III sites of the vector pECBAC1. The two libraries contain a total of 55,296 BAC clones arrayed in 144 384-well microtiter plates and correspond to 13.36 haploid genome equivalents. The combined libraries have a greater than 99% probability of containing any single-copy sequence. Screening high-density arrays of the libraries with probes for female-specific markers and sex-related genes generated between 4–46 primary positive clones per probe. Thus, the two BAC libraries of C . semilaevis provided a readily useable platform for genomics research, illustrated by the isolation of sex determination gene(s).
ISSN:1436-2228
1436-2236
DOI:10.1007/s10126-009-9242-x