Nucleotide sequence of the coat protein gene of pelargonium leaf curl virus and comparison of the deduced coat protein amino acid sequence with those of other tombusviruses

The sequence of 1,787 nucleotides (nts) in the genomic RNA of pelargonium leaf curl virus (PLCV) was determined. It included the entire coat protein (cp) gene (nts 585 to 1,754), 558 nts of the 3' end of the putative RNA polymerase gene, 26 nts of an intercistronic region between the two genes...

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Bibliographic Details
Published in:Archives of virology 1993-01, Vol.129 (1-4), p.349-356
Main Authors: BACHMANN, Y. L, MAISS, E, COMMANDEUR, U, BREYEL, E, TIMPE, U, KOENIG, R
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Biological and medical sciences
Capsid - chemistry
Capsid - genetics
DNA, Viral
Escherichia coli
Fundamental and applied biological sciences. Psychology
Genetics
Microbiology
Molecular Sequence Data
pelargonium leaf curl virus
Plant Viruses - genetics
Plants - microbiology
Sequence Alignment
tombusvirus
Virology
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Summary:The sequence of 1,787 nucleotides (nts) in the genomic RNA of pelargonium leaf curl virus (PLCV) was determined. It included the entire coat protein (cp) gene (nts 585 to 1,754), 558 nts of the 3' end of the putative RNA polymerase gene, 26 nts of an intercistronic region between the two genes and 33 nts downstream of the stop codon of the cp gene. The cp gene was cloned into the expression vector pET8c and expressed in E. coli. The deduced cp amino acid sequence of PLCV was compared with those of five other tombusviruses. The closer the degree of serological relatedness between two viruses, the more similarity was found in their cp amino acid sequences not only in the protruding domains, but also in their random and shell domains and in the arm regions. Nucleic acid hybridization tests, cp amino acid comparisons and serological tests all suggest the same order of sequence for the relationships in the tombusvirus group.
ISSN:0304-8608
1432-8798
DOI:10.1007/BF01316911